Bergelson J M, Cunningham J A, Droguett G, Kurt-Jones E A, Krithivas A, Hong J S, Horwitz M S, Crowell R L, Finberg R W
Division of Infectious Diseases, Dana-Farber Cancer Institute, Harvard Medical School, Boston, MA 02115, USA.
Science. 1997 Feb 28;275(5304):1320-3. doi: 10.1126/science.275.5304.1320.
A complementary DNA clone has been isolated that encodes a coxsackievirus and adenovirus receptor (CAR). When transfected with CAR complementary DNA, nonpermissive hamster cells became susceptible to coxsackie B virus attachment and infection. Furthermore, consistent with previous studies demonstrating that adenovirus infection depends on attachment of a viral fiber to the target cell, CAR-transfected hamster cells bound adenovirus in a fiber-dependent fashion and showed a 100-fold increase in susceptibility to virus-mediated gene transfer. Identification of CAR as a receptor for these two unrelated and structurally distinct viral pathogens is important for understanding viral pathogenesis and has implications for therapeutic gene delivery with adenovirus vectors.
已分离出一个编码柯萨奇病毒和腺病毒受体（CAR）的互补DNA克隆。用CAR互补DNA转染后，原本不具有感染性的仓鼠细胞变得易受柯萨奇B病毒的附着和感染。此外，与先前证明腺病毒感染依赖于病毒纤维附着到靶细胞的研究一致，转染了CAR的仓鼠细胞以纤维依赖的方式结合腺病毒，并且对病毒介导的基因转移的敏感性增加了100倍。将CAR鉴定为这两种不相关且结构不同的病毒病原体的受体，对于理解病毒发病机制很重要，并且对腺病毒载体的治疗性基因递送具有重要意义。
