D'Hondt K, Stack S, Gutteridge S, Vandekerckhove J, Krebbers E, Gal S
Plant Genetic Systems, Gent, Belgium.
Plant Mol Biol. 1997 Jan;33(1):187-92. doi: 10.1023/a:1005794917200.
Active aspartic proteinase is isolated from Brassica napus seeds and the peptide sequence is used to generate primers for PCR. We present here cDNA and genomic clones for aspartic proteinases from the closely related Brassicaceae Arabidopsis thaliana and Brassica napus. The Arabidopsis cDNA represents a single gene, while Brassica has at least 4 genes. Like other plant aspartic proteases, the two Brassicaceae enzymes contain an extra protein domain of about 100 amino acids relative to the mammalian forms. The intron/exon arrangement in the Brassica genomic clone is significantly different from that in mammalian genes. As the proteinase is isolated from seeds, the same tissue where 2S albumins are processed, this implies expression of one of the aspartic proteinase genes there.
从甘蓝型油菜种子中分离出活性天冬氨酸蛋白酶，并利用其肽序列生成用于聚合酶链反应（PCR）的引物。我们在此展示了来自近缘十字花科植物拟南芥和甘蓝型油菜的天冬氨酸蛋白酶的互补脱氧核糖核酸（cDNA）和基因组克隆。拟南芥的cDNA代表一个单一基因，而甘蓝型油菜至少有4个基因。与其他植物天冬氨酸蛋白酶一样，这两种十字花科植物的酶相对于哺乳动物形式含有一个约100个氨基酸的额外蛋白质结构域。甘蓝型油菜基因组克隆中的内含子/外显子排列与哺乳动物基因中的显著不同。由于蛋白酶是从种子中分离出来的，而种子也是2S白蛋白加工的同一组织，这意味着其中一个天冬氨酸蛋白酶基因在那里表达。
