Tanaka J, Murate M, Wang C Z, Seino S, Iwanaga T
Laboratory of Anatomy, Postgraduate School of Veterinary Medicine, Hokkaido University, Sapporo, Japan.
Arch Histol Cytol. 1996 Dec;59(5):485-90. doi: 10.1679/aohc.59.485.
RNA blot analysis has shown that an ionotropic ATP receptor P2X4 is widely distributed, as compared with other P2X subtypes. Cellular distribution of the P2X4 ATP receptor mRNA was investigated by in situ hybridization technique in the brain and non-neuronal organs. In the brain, transcripts of the P2X4 were widely distributed in various regions, with the highest signals in the cerebellar Purkinje cells and granule cells. In other organs, intense and selective expression was detected in seminiferous tubules of the testis and intestinal crypts of the colon, suggesting an involvement of ATP in spermatogenesis and intestinal secretion. A low but significant level of the P2X4 mRNA was detected in the adenohypophysis and the cortex of the adrenal gland. The tissue- or cell-specific expression of the P2X4 mRNA is of importance to understand the functional roles of the purinergic transmission system.
RNA印迹分析表明，与其他P2X亚型相比，离子型ATP受体P2X4分布广泛。采用原位杂交技术研究了P2X4 ATP受体mRNA在脑和非神经器官中的细胞分布。在脑中，P2X4的转录本广泛分布于各个区域，在小脑浦肯野细胞和颗粒细胞中信号最强。在其他器官中，在睾丸的生精小管和结肠的肠隐窝中检测到强烈且选择性的表达，提示ATP参与精子发生和肠道分泌。在腺垂体和肾上腺皮质中检测到低水平但显著的P2X4 mRNA。P2X4 mRNA的组织或细胞特异性表达对于理解嘌呤能传递系统的功能作用具有重要意义。
