单管巢式聚合酶链反应在沙眼衣原体感染诊断中的应用

One-tube nested polymerase chain reaction in the diagnosis of Chlamydia trachomatis infections.

作者信息

Zanetti S, Sechi L, Pinna A, Sanna A, Usai D, Carta F, Fadda G

机构信息

Institute of Microbiology and Virology, University of Sassari, Italy.

出版信息

New Microbiol. 1997 Jan;20(1):29-34.

PMID:9037666

Abstract

A one-tube nested polymerase chain reaction (PCR) using primers derived from the major outer membrane protein gene sequence of Chlamydia trachomatis was compared with McCoy cell culture for the detection of the micro-organism in clinical specimens. Of 110 urogenital and ocular specimens, 21 were positive by culture and 25 by nested PCR; all 21 culture-positive specimens were also positive by nested PCR. After resolution of discrepant specimens as true-positive, sensitivity was 100% for nested PCR and 84% for cell culture, while specificity was 100% for both tests. One-tube nested PCR appears to be a rapid, highly sensitive and specific technique for diagnosing C. trachomatis infections.

摘要

将使用源自沙眼衣原体主要外膜蛋白基因序列的引物进行的单管巢式聚合酶链反应（PCR）与McCoy细胞培养法相比较，以检测临床标本中的该微生物。在110份泌尿生殖系统和眼部标本中，21份经培养呈阳性，25份经巢式PCR呈阳性；所有21份培养阳性标本经巢式PCR也呈阳性。在将有差异的标本判定为真阳性后，巢式PCR的敏感性为100%，细胞培养的敏感性为84%，而两种检测方法的特异性均为100%。单管巢式PCR似乎是一种诊断沙眼衣原体感染的快速、高度敏感且特异的技术。