采用酶免疫测定和DNA扩增技术检测尿液中的沙眼衣原体。
Detection of Chlamydia trachomatis in urine using enzyme immunoassay and DNA amplification.
作者信息
Rasmussen S J, Smith-Vaughan H, Nelson M, Chan S W, Timms P, Capon A G
机构信息
Centre for Molecular Biotechnology, School of Life Science, Queensland University of Technology, Brisbane, Australia.
出版信息
Mol Cell Probes. 1993 Dec;7(6):425-30. doi: 10.1006/mcpr.1993.1063.
The suitability of urine specimens for the detection of Chlamydia trachomatis infections in men was assessed. Urethral swabs from 301 patients were cultured for C. trachomatis, and the results were compared with results obtained from Chlamydiazyme. The results of 298 specimens were also compared with results obtained from PCR analysis of first-void urine specimens. The sensitivity of confirmed Chlamydiazyme analysis was 93% and the specificity was greater than 99% compared with culture. The sensitivity of the PCR method was 100% compared with culture. Chlamydia trachomatis was detected by PCR in an additional three specimens from which C. trachomatis could not be cultured. Urine appears to be an appropriate specimen for the detection of C. trachomatis antigens and nucleic acids.
对男性尿液标本用于检测沙眼衣原体感染的适用性进行了评估。对301例患者的尿道拭子进行沙眼衣原体培养，并将结果与衣原体酶检测结果进行比较。还将298份标本的结果与首次晨尿标本的PCR分析结果进行比较。与培养法相比，确诊的衣原体酶分析的敏感性为93%，特异性大于99%。与培养法相比，PCR方法的敏感性为100%。在另外3份无法培养出沙眼衣原体的标本中，通过PCR检测到了沙眼衣原体。尿液似乎是检测沙眼衣原体抗原和核酸的合适标本。
Zotero 插件