Calderaro A, Dettori G, Grillo R, Spinetti A P, Storchi Incerti S, Cattani P, Chezzi C
Institute of Microbiology, Faculty of Medicine and Surgery, University of Parma, Italy.
New Microbiol. 1997 Jan;20(1):35-45.
This study aimed at making a comparative assessment of the growth of pure cultures of Human Intestinal Spirochaetes (HIS) in a control medium without antibiotics and in media including antibiotics (spectinomycin, rifampin, colistin, polymyxin B, amphotericin B, vancomycin, spiramycin) whose use had been indicated in the literature in connection with the isolation of HIS or animal intestinal spirochaetes. All the strains of HIS tested grew in media to which appropriate concentrations of those drugs had been added giving a final number of CFU/ml +/- 10 times the number of CFU/ml observed in the control medium. These results indicate that a selective medium to be used for the isolation of HIS may include appropriate concentrations of one or more of the following antibiotics: spectinomycin (100-1,000 micrograms/ml), rifampin (10-30 micrograms/ml), vancomycin (6.25 micrograms/ml), colistin (6.25 micrograms/ml), polymyxin B (5 micrograms/ml), spiramycin (1-10 micrograms/ml) and amphotericin B (0.05-35 micrograms/ml).
本研究旨在对人类肠道螺旋体（HIS）纯培养物在不含抗生素的对照培养基以及含有抗生素（壮观霉素、利福平、黏菌素、多黏菌素B、两性霉素B、万古霉素、螺旋霉素）的培养基中的生长情况进行比较评估，这些抗生素在文献中曾被提及与HIS或动物肠道螺旋体的分离有关。所有测试的HIS菌株在添加了适当浓度这些药物的培养基中均能生长，最终的菌落形成单位（CFU）/毫升数量为对照培养基中观察到的CFU/毫升数量的±10倍。这些结果表明，用于分离HIS的选择性培养基可包含以下一种或多种抗生素的适当浓度：壮观霉素（100 - 1000微克/毫升）、利福平（10 - 30微克/毫升）、万古霉素（6.25微克/毫升）、黏菌素（6.25微克/毫升）、多黏菌素B（5微克/毫升）、螺旋霉素（1 - 10微克/毫升）和两性霉素B（0.05 - 35微克/毫升）。
