Calderaro A, Dettori G, Grillo R, Cattani P, Chezzi C
Institute of Microbiology, Faculty of Medicine and Surgery, University of Parma, Italy.
New Microbiol. 1997 Jan;20(1):47-54.
The growth of pure cultures of 24 human intestinal spirochaetes (HIS) was analysed comparatively in six selective media with antibiotics and in a control medium without antibiotics. The selective media SR and SP were the only two media among the six tested which allowed the growth of all the strains studied. These media contained spectinomycin (400 micrograms/ml) and rifampin (30 micrograms/ml) (SR), and spectinomycin (400 micrograms/ml) and polymyxin B (5 micrograms/ml) (SP), respectively. Moreover, most of the strains tested showed in these two media a number of CFU/ml equal to, or, for a few strains, not more than ten-fold lower than that observed in the control medium. The other four selective media tested were: SRVC (spectinomycin 200 micrograms/ml; rifampin 12.5 micrograms/ml; vancomycin 6.25 micrograms/ml; colistin 6.25 micrograms/ml), CSp (colistin 6.25 micrograms/ml; spiramycin 25 micrograms/ml), SRSp (spectinomycin 200 micrograms/ml; rifampin 12.5 micrograms/ml; spiramycin 25 micrograms/ml) and SRVCSp (spectinomycin 200 micrograms/ml; rifampin 12.5 micrograms/ml; vancomycin 6.25 micrograms/ml; colistin 6.25 micrograms/ml; spiramycin 25 micrograms/ml). The growth of most of the spirochaetes was strongly reduced in the media SRVC, CSp, SRSp and SRVCSp. Furthermore, several of the 24 HIS examined did not grow in the medium SRVC (3 spirochaetes, 11%), CSp (15 spirochaetes, 62%), SRSp (17 spirochaetes, 70%), SRVCSp (19 spirochaetes, 79%). The results reported in this paper indicate that the media SR and SP, of the six selective media tested, may profitably be used in the isolation of HIS as they did not significantly affect the growth of the HIS tested.
在六种含抗生素的选择性培养基和一种不含抗生素的对照培养基中，对24株人肠道螺旋体（HIS）的纯培养物生长情况进行了比较分析。选择性培养基SR和SP是所测试的六种培养基中仅有的两种能使所有研究菌株生长的培养基。这两种培养基分别含有壮观霉素（400微克/毫升）和利福平（30微克/毫升）（SR），以及壮观霉素（400微克/毫升）和多粘菌素B（5微克/毫升）（SP）。此外，大多数测试菌株在这两种培养基中的每毫升菌落形成单位（CFU/ml）数量与对照培养基中观察到的数量相等，或者对于少数菌株来说，比对照培养基中观察到的数量低不超过十倍。测试的其他四种选择性培养基为：SRVC（壮观霉素200微克/毫升；利福平12.5微克/毫升；万古霉素6.25微克/毫升；黏菌素6.25微克/毫升）、CSp（黏菌素6.25微克/毫升；螺旋霉素25微克/毫升）、SRSp（壮观霉素200微克/毫升；利福平12.5微克/毫升；螺旋霉素25微克/毫升）和SRVCSp（壮观霉素200微克/毫升；利福平12.5微克/毫升；万古霉素6.25微克/毫升；黏菌素6.25微克/毫升；螺旋霉素25微克/毫升）。在SRVC、CSp、SRSp和SRVCSp培养基中，大多数螺旋体的生长受到强烈抑制。此外，所检测的24株HIS中有几株在SRVC培养基（3株螺旋体，11%）、CSp培养基（15株螺旋体，62%）、SRSp培养基（17株螺旋体，70%）、SRVCSp培养基（19株螺旋体，79%）中不生长。本文报道的结果表明，在所测试的六种选择性培养基中，培养基SR和SP可有效地用于分离HIS，因为它们对所测试的HIS生长没有显著影响。
