从波罗蜜种子中提取的一种诱导中性粒细胞迁移的凝集素（KM+）的结晶及初步晶体学数据

Crystallization and preliminary crystallographic data of a neutrophil migration-inducing lectin (KM+) extracted from the seed of Artocarpus integrifolia.

作者信息

de Oliveira P S, Garratt R C, Mascarenhas Y P, Beltramini L M, Roque-Barreira M C, Harvey I, Oliva G

机构信息

Instituto de Física de São Carlos, Universidade de São Paulo, Brazil.

出版信息

Proteins. 1997 Jan;27(1):157-9. doi: 10.1002/(sici)1097-0134(199701)27:1<157::aid-prot16>3.3.co;2-u.

DOI:10.1002/(sici)1097-0134(199701)27:1<157::aid-prot16>3.3.co;2-u

PMID:9037721

Abstract

The tetrameric KM+ lectin from the seeds of Artocarpus integrifolia has, when compared to other plant lectins, the singular property of directly inducing neutrophil migration into the peritoneal cavity or into the air pouch of rats. This protein crystals have been grown and they belong to the orthorhombic system with space group C222(1). The unit cell parameters are a = 54.4 A, b = 127.9 A and c = 99.8 A. A native diffraction dataset to 2.8 A was collected and an analysis of the self-rotation function has shown the presence of only one independent non-crystallographic 2-fold axis orthogonal to the crystal b-axis, compatible with a dimer in the asymmetric unit.

摘要

与其他植物凝集素相比，波罗蜜种子中的四聚体KM+凝集素具有直接诱导中性粒细胞迁移至大鼠腹腔或气袋的独特特性。该蛋白质晶体已生长出来，属于正交晶系，空间群为C222(1)。晶胞参数为a = 54.4 Å，b = 127.9 Å，c = 99.8 Å。收集了分辨率为2.8 Å的天然衍射数据集，对自旋转函数的分析表明，仅存在一个与晶体b轴正交的独立非晶体学2重轴，这与不对称单元中的二聚体相符。