Rao K N, Gurjar M M, Gaikwad S M, Khan M I, Suresh C G
Division of Biochemical Sciences, National Chemical Laboratory, Pune 411008, India.
Acta Crystallogr D Biol Crystallogr. 1999 Jun;55(Pt 6):1204-5. doi: 10.1107/s0907444999003571.
The basic lectin from Artocarpus hirsuta specific towards methyl alpha-galactose has been purified and crystallized using the hanging-drop vapour-diffusion method with ammonium sulfate as precipitant. Three different crystal forms, orthorhombic I, orthorhombic II and hexagonal, were grown under the same crystallization conditions. The orthorhombic forms belonged to space group P212121 with unit-cell dimensions a = 92.9, b = 99.8, c = 166. 2 A and a = 89.9, b = 121.9, c = 131.6 A, respectively. The unit-cell dimensions of the hexagonal form were a = b = 84.1 and c = 271.7 A and the space group was P6122.
已使用硫酸铵作为沉淀剂，通过悬滴气相扩散法对来自糙叶桂木、对α-甲基半乳糖具有特异性的基础凝集素进行了纯化和结晶。在相同的结晶条件下生长出了三种不同的晶体形式，即正交晶系I、正交晶系II和六方晶系。正交晶系形式分别属于空间群P212121，其晶胞参数为a = 92.9、b = 99.8、c = 166.2 Å和a = 89.9、b = 121.9、c = 131.6 Å。六方晶系形式的晶胞参数为a = b = 84.1和c = 271.7 Å，空间群为P6122。
