Manoj N, Srinivas V R, Satish B, Singha N C, Suguna K
Molecular Biophysics Unit, Indian Institute of Science, Bangalore 560 012, India.
Acta Crystallogr D Biol Crystallogr. 1999 Feb;55(Pt 2):564-5. doi: 10.1107/s0907444998013018.
The acidic lectin (WBAII) from the winged bean (Psophocarpus tetragonolobus) binds to the H-antigenic determinant on human erythrocytes and to the T-antigenic disaccharide Gal-beta1,3-GalNAc. Two crystal forms of WBAII were obtained in the presence of methyl-alpha-D-galactose. Form I belongs to space group R3 with unit-cell dimensions a = b = 182.11, c = 44.99 A and has one dimer in the asymmetric unit. Form II belongs to space group C2 with unit-cell dimensions a = 135.36, b = 127.25, c = 139.98 A, beta = 95. 9 degrees and has four dimers in the asymmetric unit. Intensity data were collected to 3.0 A and to 3.5 A from crystals of form I and II, respectively. The structures were solved by the molecular-replacement method using the coordinates of the basic form of winged bean lectin.
来自四棱豆（Psophocarpus tetragonolobus）的酸性凝集素（WBAII）可与人红细胞上的H抗原决定簇以及T抗原二糖Gal-β1,3-GalNAc结合。在甲基-α-D-半乳糖存在的情况下获得了WBAII的两种晶体形式。晶型I属于空间群R3，晶胞参数a = b = 182.11，c = 44.99 Å，不对称单元中有一个二聚体。晶型II属于空间群C2，晶胞参数a = 135.36，b = 127.25，c = 139.98 Å，β = 95.9°，不对称单元中有四个二聚体。分别从晶型I和II的晶体收集了分辨率为3.0 Å和3.5 Å的强度数据。使用四棱豆凝集素基本形式的坐标通过分子置换法解析了结构。
