Zamierowski M M, Wagner C
J Nutr. 1977 Oct;107(10):1937-45. doi: 10.1093/jn/107.10.1937.
The effect of experimental folacin deficiency on the uptake and distribution of radioactive folic acid in the rat was investigated. Less radioactivity was taken up by livers of deficient rats than controls 24 hours after intraperitoneal injection of [3H]-folic acid, although more radioactivity was incorporated by the brain and kidneys of deficient rats. The distribution of radioactivity among the three folacin-binding proteins of rat liver cytosol and the binding protein of mitochondria was also studied. In deficiency, very little radioactivity was incorporated into cytosol binding proteins I and II, while more radioactivity was incorporated into cytosol binding protein II and the mitochondrial binding protein. A decrease in the endogenous folacin associated with all protein-bound and free forms was seen in deficiency with the major decrease coming at the expense of unbound folacin, and cytosol binding protein I. This latter protein may have a primary storage role in the liver.
研究了实验性叶酸缺乏对大鼠体内放射性叶酸摄取和分布的影响。腹腔注射[3H] - 叶酸24小时后，缺乏叶酸的大鼠肝脏摄取的放射性比对照组少，尽管缺乏叶酸的大鼠脑和肾脏摄取的放射性更多。还研究了放射性在大鼠肝脏细胞质的三种叶酸结合蛋白和线粒体结合蛋白中的分布。在叶酸缺乏状态下，细胞质结合蛋白I和II几乎没有摄取放射性，而细胞质结合蛋白II和线粒体结合蛋白摄取的放射性更多。叶酸缺乏时，与所有蛋白质结合形式和游离形式相关的内源性叶酸减少，主要减少的是未结合叶酸和细胞质结合蛋白I。后一种蛋白可能在肝脏中起主要储存作用。
