Roman E, Moreno C
MRC Tuberculosis and Related Infections Unit, Royal Postgraduate Medical School, Hammersmith Hospital, London, UK.
Immunology. 1997 Jan;90(1):52-6. doi: 10.1046/j.1365-2567.1997.00136.x.
Four synthetic peptides bearing dominant CD4+ T-cell epitopes of the 19,000 and 38,000 MW proteins of Mycobacterium tuberculosis were used to provoke a delayed-type hypersensitivity (DTH) reaction in mice previously immunized with recombinant 19,000 and 38,000 MW proteins. It was found that an effective enhancement of the DTH reaction was obtained if the peptides were administered as a complex with M. tuberculosis hsp 70 protein. The increase in reactivity was not obtained when hsp 70 and peptide were co-injected at the same site, but not in complex, or when the specific peptide was displaced from the complex by an irrelevant peptide with high capacity to bind hsp 70. One of the antigenic peptides whose capacity to complex with hsp 70 is low, failed to show the enhancement of DTH when injected together with hsp 70.
四种带有结核分枝杆菌19,000和38,000 MW蛋白主要CD4 + T细胞表位的合成肽，用于在先前用重组19,000和38,000 MW蛋白免疫的小鼠中引发迟发型超敏反应（DTH）。结果发现，如果将这些肽与结核分枝杆菌hsp 70蛋白作为复合物给药，则可有效增强DTH反应。当hsp 70和肽在同一部位共同注射但不是形成复合物时，或者当特异性肽被具有高结合hsp 70能力的无关肽从复合物中置换时，未获得反应性的增加。其中一种与hsp 70形成复合物能力低的抗原肽，与hsp 70一起注射时未显示DTH增强。
