Minden P, Houghten R A, Spear J R, Shinnick T M
Infect Immun. 1986 Sep;53(3):560-4. doi: 10.1128/iai.53.3.560-564.1986.
Monoclonal antibodies directed to Mycobacterium bovis BCG (BCG) and to M. tuberculosis H37Rv (H37Rv) were used in conjunction with affinity chromatography to prepare a mycobacterial component which was designated BCG-a. A synthetic peptide antigen was prepared based on the amino acid sequence of BCG-a and was designated BCG-a-P. Significant immunological similarities were found between BCG-a-P and antigens in extracts of BCG and H37Rv but not between BCG-a-P and antigens of nontuberculous mycobacteria. An enzyme-linked immunosorbent assay detected antibodies to BCG-a-P in sera from rabbits that had been immunized with BCG and H37Rv sonicates. In Western blot analysis, antibodies to BCG-a-P reacted to 10,000-molecular-weight components of extracts of BCG and H37Rv. Delayed cutaneous hypersensitivity reactions to BCG-a-P were elicited in guinea pigs immunized with sonicates of BCG and H37Rv but were weak or nonexistent in unimmunized animals or in animals immunized with sonicates of nontuberculous mycobacteria. This study points out the feasibility of using monoclonal antibodies to prepare and characterize synthetic mycobacterial peptides with a potential for immunodiagnostic purposes.
将针对牛分枝杆菌卡介苗（BCG）和结核分枝杆菌H37Rv（H37Rv）的单克隆抗体与亲和色谱法结合使用，制备了一种名为BCG-a的分枝杆菌成分。基于BCG-a的氨基酸序列制备了一种合成肽抗原，并将其命名为BCG-a-P。发现BCG-a-P与BCG和H37Rv提取物中的抗原之间存在显著的免疫学相似性，但BCG-a-P与非结核分枝杆菌的抗原之间不存在这种相似性。酶联免疫吸附测定法检测到用BCG和H37Rv超声裂解物免疫的兔血清中存在针对BCG-a-P的抗体。在蛋白质印迹分析中，针对BCG-a-P的抗体与BCG和H37Rv提取物的10,000分子量成分发生反应。在用BCG和H37Rv超声裂解物免疫的豚鼠中引发了对BCG-a-P的迟发型皮肤超敏反应，但在未免疫的动物或用非结核分枝杆菌超声裂解物免疫的动物中，这种反应较弱或不存在。本研究指出了使用单克隆抗体制备和鉴定具有免疫诊断潜力的合成分枝杆菌肽的可行性。
