Graczyk T K, Cranfield M R, Fayer R
Department of Molecular Microbiology and Immunology, Johns Hopkins University, School of Hygiene and Public Health, Baltimore, MD 21205, USA.
Parasitol Res. 1997;83(2):121-5. doi: 10.1007/s004360050221.
The cellulose-acetate membrane (CAM)-filter dissolution method implemented into a Millipore Glass Microanalysis system was used for recovery of Cryptosporidium parvum oocysts seeded into 25 l of drinking water in polyethylene carboy aspirator bottles. CAM-entrapped oocysts were detected by immunofluorescence microscopy. From 65 to 94 oocysts/l (mean 75 oocysts/l), 34.7% overall of the inoculated oocysts, were unrecovered as determined after the water had been drained from the bottle, rinsed with 1 l of eluting fluid (EF), and CAM-filtered. Efficiency rates of oocyst recovery ranged from 24.0% to 64.0% (mean 44.1%), without the use of EF and from 72.1% to 82.3% (mean 78.8%) when EF was used. To ensure a high recovery efficiency of Cryptosporidium oocysts from sampled water by the CAM-filter dissolution method, it is recommended that 1 l of EF per 25 l of water be used.
将纤维素醋酸酯膜（CAM）过滤溶解法应用于密理博玻璃微量分析系统，用于回收接种于25升聚乙烯大玻璃瓶饮用水中的微小隐孢子虫卵囊。通过免疫荧光显微镜检测CAM截留的卵囊。从每升65至94个卵囊（平均每升75个卵囊），即接种卵囊总数的34.7%，在水从瓶中排出、用1升洗脱液（EF）冲洗并经CAM过滤后未被回收。在不使用EF的情况下，卵囊回收率为24.0%至64.0%（平均44.1%），使用EF时回收率为72.1%至82.3%（平均78.8%）。为确保通过CAM过滤溶解法从采样水中高效回收隐孢子虫卵囊，建议每25升水使用1升EF。
