兔眼角膜上皮伤口愈合过程中局部应用P物质的研究

Topical substance P and corneal epithelial wound closure in the rabbit.

作者信息

Kingsley R E, Marfurt C F

机构信息

Indiana University School of Medicine, South Bend Center for Medical Education, University of Notre Dame, IN 46556, USA.

出版信息

Invest Ophthalmol Vis Sci. 1997 Feb;38(2):388-95.

PMID:9040472

Abstract

PURPOSE

The authors determined the effect of topically applied substance P (SP) on the rate of corneal epithelial wound closure in the rabbit.

METHODS

Uniform circular lesions, 6.5 mm in diameter, were made bilaterally in the corneal epithelium of 24 rabbits using N-heptanol. Substance P was applied repeatedly to one eye, and the SP1-7 fragment was applied to the contralateral (control) eye until wound closure was obtained. Three concentrations of peptide solution (5 x 10(-5) M, 5 x 10(-4) M, and 5 x 10(-3) M) were tested in separate groups of eight animals each. An additional eight animals received topical applications (5 x 10(-7) M) of the neurokinin-1 (NK1)-specific SP receptor antagonist CP-99,994-01 or its ineffective enantiomer CP-100,263-01. The mean rates of wound healing for each group of experimental and control eyes were determined by linear regression and analyzed by analysis of variance.

RESULTS

There were no statistically significant differences in the mean rates of wound closure (range, 0.083 to 0.106 mm/hour) between experimental- and control-treated corneas for any of the four groups tested.

CONCLUSIONS

The topical application of SP or its NK1 receptor antagonist has no significant effect on the rate of corneal epithelial wound closure in the rabbit.

摘要

目的

作者测定了局部应用P物质（SP）对兔角膜上皮伤口闭合速率的影响。

方法

使用正庚醇在24只兔的双侧角膜上皮制造直径6.5mm的均匀圆形损伤。将P物质反复应用于一只眼，将SP1 - 7片段应用于对侧（对照）眼，直至伤口闭合。在每组8只动物的单独组中测试三种浓度的肽溶液（5×10⁻⁵M、5×10⁻⁴M和5×10⁻³M）。另外8只动物接受神经激肽-1（NK1）特异性SP受体拮抗剂CP - 99,994 - 01或其无效对映体CP - 100,263 - 01的局部应用（5×10⁻⁷M）。通过线性回归确定每组实验眼和对照眼的平均伤口愈合速率，并通过方差分析进行分析。