剑尾鱼（硬骨鱼纲：花鳉科）RPS15基因的特征分析与定位

Characterization and mapping of the Xiphophorus maculatus (Teleostei: Poeciliidae) RPS15 gene.

作者信息

Walter R B, Obermoeller R D, Moore D D, Lacson J M, Della Coletta L, McEntire B B, Morizot D C, Nairn R S

机构信息

Department of Biology, Southwest Texas State University, San Marcos, USA.

出版信息

Cytogenet Cell Genet. 1996;75(2-3):140-4. doi: 10.1159/000134465.

DOI:10.1159/000134465

PMID:9040780

Abstract

We have determined the nucleotide sequence and gene map location of the Xiphophorus maculatus homologue of RPS15 (ribosomal protein S15, alias RIG). The Xiphophorus RPS15 cDNA encodes 145 amino acids, which show 94% identity compared to deduced mammalian and avian RPS15 amino acid sequences. At the nucleotide level, 84% sequence identity is maintained between the fish and human gene, while homologous amphibian and avian sequences show about 80% nucleotide identity compared to the Xiphophorus sequence. Nucleotide identity substantially decreases when the fish gene is compared to Arabidopsis S15 (64%) and yeast S21 (55%) genes. Genetic linkage analysis of an RPS15 restriction fragment length polymorphism in backcross hybrids generated from the cross X. helleri x (X. maculatus Jp 163 B x X. helleri) demonstrated linkage of Xiphophorus RPS15 to the EGFR, UMPK and YES loci in Xiphophorus Linkage Group VI.

摘要

我们已经确定了剑尾鱼（Xiphophorus maculatus）核糖体蛋白S15（RPS15，别名RIG）同源物的核苷酸序列和基因图谱位置。剑尾鱼RPS15 cDNA编码145个氨基酸，与推导的哺乳动物和禽类RPS15氨基酸序列相比，同一性为94%。在核苷酸水平上，鱼类和人类基因之间保持84%的序列同一性，而同源的两栖类和禽类序列与剑尾鱼序列相比，核苷酸同一性约为80%。当将鱼类基因与拟南芥S15（64%）和酵母S21（55%）基因进行比较时，核苷酸同一性显著降低。对由赫氏剑尾鱼（X. helleri）与（斑剑尾鱼Jp 163 B×赫氏剑尾鱼）杂交产生的回交杂种中RPS15限制性片段长度多态性进行的遗传连锁分析表明，剑尾鱼RPS15与剑尾鱼连锁群VI中的表皮生长因子受体（EGFR）、尿苷一磷酸激酶（UMPK）和Yes基因座连锁。