Huang H, Zhang X, Chen Y, Cui Y
Nanjing Normal University.
Yi Chuan Xue Bao. 1996;23(5):338-42.
An experimental method of fluorescence in situ hybridization (FISH) with conventionally G-band chromosomes was developed to analyze the complex chromosome rearrangements of patients. In order to obtain optimal results of both G-banding and fluorescence signals, it was essential that G-banded chromosomes were fixed with formaldehyde prior to fluorescence in situ hybridization. The karyotype of a patient with spontaneous abortion might be described as 46, XX, t(1; 5; 12) (1pter-->1q25 : : 12q24-->12qter; 5qter-->5p11 : : 1q25-->1qter; 12pter-->12q24 : : 5p11-->5pter) by the analysis of conventional cytogenetics. However, her karyotype should be identified as 46, XX, t(1; 5; 12) (1pter-->1q23 : : 12q22-->12qter; 5qter-->5p11 : : 1q25-->1qter; 12pter-->12q22 : : 1q23-->1q25 : : 5p11-->5pter) with this newly established method. This investigation indicates that this technique described above is a powerful tool to detect complex chromosome rearrangements of patients.
一种将荧光原位杂交（FISH）与传统G带染色体相结合的实验方法被开发出来，用于分析患者复杂的染色体重排。为了同时获得G带和荧光信号的最佳结果，在荧光原位杂交之前，用甲醛固定G带染色体至关重要。通过传统细胞遗传学分析，一名自然流产患者的核型可能被描述为46, XX, t(1; 5; 12) (1pter-->1q25 : : 12q24-->12qter; 5qter-->5p11 : : 1q25-->1qter; 12pter-->12q24 : : 5p11-->5pter)。然而，用这种新建立的方法，她的核型应被确定为46, XX, t(1; 5; 12) (1pter-->1q23 : : 12q22-->12qter; 5qter-->5p11 : : 1q25-->1qter; 12pter-->12q22 : : 1q23-->1q25 : : 5p11-->5pter)。这项研究表明，上述技术是检测患者复杂染色体重排的有力工具。
