Brocchi E, Zhang G, Knowles N J, Wilsden G, McCauley J W, Marquardt O, Ohlinger V F, De Simone F
Institute for Animal Health, Pirbright Laboratory, Woking, Surrey, United Kingdom.
Epidemiol Infect. 1997 Feb;118(1):51-61. doi: 10.1017/s0950268896007170.
Viruses from the recent epidemic of swine vesicular disease (SVD) in Europe have been isolated and characterized by antigenic and genetic methods to examine the likely epidemiological origins of the disease. Antigenic analysis was performed on 77 SVD viruses (SVDV) isolated in Europe between 1966 and 1994 using two panels of monoclonal antibodies (MAb) in a trapping ELISA. Genetic analysis of 33 of the SVD viruses by reverse transcription-polymerase chain-reaction (RT-PCR) amplification and nucleotide sequencing of the ID (VP1) coding region was also performed. Comparison of the nucleotide sequences with each other and with three other previously published SVDV sequences revealed four distinct groups which correlated exactly with the results of the pattern of reactivity with MAbs. The first group consisted solely of the earliest SVD virus isolated (ITL/1/66) while the second group comprised viruses present in Europe and Japan between 1972 and 1981. The third group consisted of viruses isolated from outbreaks of SVD in Italy between December 1988 and June 1992. Viruses isolated between 1987 and 1994 from Romania, the Netherlands, Italy and Spain formed a fourth group. The genetic and antigenic similarity of the most recent virus isolates from Western Europe to a virus isolated in Romania 5 years previously suggests that the possible origin of the recent epidemic of swine vesicular disease in Western Europe was in Eastern Europe.
欧洲近期猪水泡病（SVD）疫情中的病毒已被分离出来，并通过抗原和基因方法进行了特征分析，以研究该疾病可能的流行病学起源。使用两组单克隆抗体（MAb）在捕获ELISA中对1966年至1994年间在欧洲分离的77株猪水泡病病毒（SVDV）进行了抗原分析。还通过逆转录聚合酶链反应（RT-PCR）扩增和ID（VP1）编码区的核苷酸测序对33株SVD病毒进行了基因分析。将核苷酸序列相互比较，并与其他三个先前发表的SVDV序列进行比较，发现了四个不同的组，这与单克隆抗体反应模式的结果完全相关。第一组仅由最早分离的SVD病毒（ITL/1/66）组成，而第二组包括1972年至1981年间在欧洲和日本出现的病毒。第三组由1988年12月至1992年6月期间从意大利SVD疫情中分离的病毒组成。1987年至1994年间从罗马尼亚、荷兰、意大利和西班牙分离的病毒形成了第四组。西欧最近分离的病毒与5年前在罗马尼亚分离的一种病毒在基因和抗原上的相似性表明，西欧近期猪水泡病疫情的可能起源在东欧。
