Chiocchia G, Smith K A
INSERM Unité 283, Hôpital COCHIN Paris, France.
Biotechniques. 1997 Feb;22(2):312-4, 316, 318. doi: 10.2144/97222st04.
A new method for detecting the expression of low-abundance mRNA molecules has been developed that combines the sensitivity of PCR, the high efficiency and specificity of reverse transcription (RT) using Tth DNA polymerase at high temperature, and the enhancement of sensitivity and specificity of nested PCR. This method is highly sensitive, reproducible and allows the detection of mRNAs in individual cells by direct RT-PCR.
一种检测低丰度mRNA分子表达的新方法已经被开发出来，该方法结合了PCR的灵敏度、利用Tth DNA聚合酶在高温下进行逆转录（RT）的高效率和特异性，以及巢式PCR对灵敏度和特异性的增强。这种方法高度灵敏、可重复，并且能够通过直接RT-PCR检测单个细胞中的mRNA。
