Mahbubani M H, Bej A K, Miller R D, Atlas R M, DiCesare J L, Haff L A
Dept. of Biology, University of Louisville, KY 40292.
Biotechniques. 1991 Jan;10(1):48-9.
A method was developed for the detection of bacterial mRNAs using reverse transcriptase followed by the polymerase chain reaction (PCR) and Southern blot analysis. The method involves brief inhibition of protein synthesis with chloramphenicol, followed by reverse transcription, PCR amplification of cDNA and Southern blot hybridization. Detection of mRNAs by reverse transcription-PCR-Southern blot analysis is orders of magnitude more sensitive than Northern blot hybridization.
开发了一种利用逆转录酶，随后进行聚合酶链反应（PCR）和Southern印迹分析来检测细菌信使核糖核酸（mRNA）的方法。该方法包括先用氯霉素短暂抑制蛋白质合成，然后进行逆转录、cDNA的PCR扩增和Southern印迹杂交。通过逆转录-聚合酶链反应- Southern印迹分析检测mRNA比Northern印迹杂交灵敏几个数量级。
