Glutamate and aspartate immunoreactivity in the reciprocal projections between the anterior thalamic nuclei and the retrosplenial granular cortex in the rat.

We have used retrograde and anterograde labelling with wheat germ agglutinin-horseradish peroxidase and immunohistochemistry with antibodies against glutamate and aspartate to examine the reciprocal connections between the anterior thalamic nuclei and the retrosplenial granular cortex in the rat, and to characterize those projection neurones that contain glutamate and/or aspartate. Injections into superficial layers of the retrosplenial granular cortex resulted in retrogradely labelled cell bodies in the anterodorsal, anteroventral, and to a lesser extent the anteromedial subnuclei. Approximately 70% of these cell bodies were also immunolabelled for glutamate or aspartate. Injections confined to deep layers (V-VI) resulted in the presence, in anterior thalamic neuropil, of anterogradely labelled fibre and terminal-like structures, many of which appeared to be immunolabelled for glutamate or aspartate. Injections into the anterior thalamic nuclei resulted in retrogradely labelled pyramidal cells in layers V-VI of the retrosplenial granular cortex. Most (90-95%) of these cells were immunolabelled for glutamate or aspartate. Thus, approximately 70% of thalamocortical and 90-95% of corticothalamic projection neurones in these circuits may use glutamate and/or aspartate as neurotransmitters.