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[采用同步扫描法分离荧光中的酪氨酸和色氨酸成分]

[Separation of the tyrosine and tryptophan components of fluorescence using synchronous scanning method].

作者信息

Vekshin I L

出版信息

Biofizika. 1996 Nov-Dec;41(6):1176-9.

PMID:9044614
Abstract

It has been shown that the method of synchronous scanning can be applied for the separation of tyrosine and tryptophan fluorescent components in solution and proteins. Choosing the shift between excitation and emission monochromators makes possible to perform also the separate detecting of "blue" and "red" components of protein fluorescence and to use the method for registration of conformational changes.

摘要

已经表明，同步扫描方法可用于分离溶液和蛋白质中的酪氨酸和色氨酸荧光成分。选择激发和发射单色仪之间的波长偏移，还可以对蛋白质荧光的“蓝色”和“红色”成分进行单独检测，并使用该方法记录构象变化。

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