Hutnik C M, Szabo A G
National Research Council of Canada, Division of Biological Sciences, Ottawa, Ontario.
Biochem Int. 1988 Apr;16(4):587-91.
The recent report of long-wavelength fluorescence of tyrosine and tryptophan solutions [Macias et al. (1987) Biochem. Int. 15, 961-969] prompted an investigation to confirm its authenticity. Similar excitation and emission spectra were recorded in the absence of any filters, but completely disappeared when a 370 nm non-fluorescent cut-off filter was placed between the excitation monochromator and the sample. These results demonstrate that excitation spectra above 400 nm and emission spectra above 550 nm originate from second order diffraction effects.
最近关于酪氨酸和色氨酸溶液长波长荧光的报告[马西亚斯等人（1987年），《生物化学国际》15卷，961 - 969页]促使人们进行一项调查以证实其真实性。在没有任何滤光片的情况下记录到了类似的激发光谱和发射光谱，但当在激发单色仪和样品之间放置一个370纳米的非荧光截止滤光片时，这些光谱完全消失了。这些结果表明，400纳米以上的激发光谱和550纳米以上的发射光谱源自二阶衍射效应。
