Hung J C, Herold T J, Gibbons R J
Nuclear Medicine, Department of Diagnostic Radiology, Mayo Clinic, Rochester, MN 55905, USA.
Nucl Med Biol. 1996 Jul;23(5):599-603. doi: 10.1016/0969-8051(96)00054-6.
Our nuclear pharmacy has reported that a failed radiochemical purity (RCP) (i.e., RCP < 90%) of 99mTc-sestamibi may be associated with the use of a first elution at later stages from a long-ingrowth time (i.e., > or = 72 h) wet-column generator. The primary purpose of this study was to evaluate the effects of 99mTc eluates from wet- and dry-column generators on the RCP of 99mTc-sestamibi under the above conditions. RCP values were found to be measurably higher and kit failure rates lower with the use of dry-column generator eluate. Using a dry-column generator eluate, Cardiolite kits were prepared with 11.10 GBq of 99mTc at 3, 4, and 5 h postelution and 5.55 GBq at 6, 10, 11, and 12 h postelution. Our data suggest that when 11.10 GBq of 99mTc from a dry-column generator with > or = 72-h ingrowth was used to prepare 99mTc-sestamibi, kit failure started to occur using 99mTc eluate at approximately 4 h postelution. When 5.55 GBq was used to reconstitute the kit, RCP failure began to occur using 99mTc eluate approximately 10 h postelution and wet-column generators; the failure rate can be reduced even further by avoiding the addition of high activities of 99mTc and long elution times.
我们的核药房报告称，99mTc-司他米比的放射化学纯度（RCP）不合格（即RCP<90%）可能与使用长生长时间（即≥72小时）的湿柱发生器后期的首次洗脱有关。本研究的主要目的是评估在上述条件下，湿柱和干柱发生器的99mTc洗脱液对99mTc-司他米比RCP的影响。结果发现，使用干柱发生器洗脱液时，RCP值明显更高，试剂盒失败率更低。使用干柱发生器洗脱液，在洗脱后3、4和5小时用11.10GBq的99mTc制备Cardiolite试剂盒，在洗脱后6、10、11和12小时用5.55GBq制备。我们的数据表明，当使用来自生长时间≥72小时的干柱发生器的11.10GBq的99mTc制备99mTc-司他米比时，使用洗脱后约4小时的99mTc洗脱液开始出现试剂盒失败。当使用5.55GBq复溶试剂盒时，使用洗脱后约10小时的99mTc洗脱液和湿柱发生器开始出现RCP不合格；通过避免添加高活度的99mTc和长洗脱时间，失败率可以进一步降低。
