• 文献检索
  • 文档翻译
  • 深度研究
  • 学术资讯
  • Suppr Zotero 插件Zotero 插件
  • 邀请有礼
  • 套餐&价格
  • 历史记录
应用&插件
Suppr Zotero 插件Zotero 插件浏览器插件Mac 客户端Windows 客户端微信小程序
定价
高级版会员购买积分包购买API积分包
服务
文献检索文档翻译深度研究API 文档MCP 服务
关于我们
关于 Suppr公司介绍联系我们用户协议隐私条款
关注我们

Suppr 超能文献

核心技术专利：CN118964589B侵权必究
粤ICP备2023148730 号-1Suppr @ 2026

文献检索

告别复杂PubMed语法，用中文像聊天一样搜索，搜遍4000万医学文献。AI智能推荐，让科研检索更轻松。

立即免费搜索

文件翻译

保留排版，准确专业，支持PDF/Word/PPT等文件格式，支持 12+语言互译。

免费翻译文档

深度研究

AI帮你快速写综述，25分钟生成高质量综述，智能提取关键信息，辅助科研写作。

立即免费体验

大肠杆菌的“蛋白酶I”在体内发挥硫酯酶的作用。

"Protease I" of Escherichia coli functions as a thioesterase in vivo.

作者信息

Cho H, Cronan J E

机构信息

Department of Microbiology, University of Illinois at Urbana-Champaign 61801.

出版信息

J Bacteriol. 1994 Mar;176(6):1793-5. doi: 10.1128/jb.176.6.1793-1795.1994.

DOI:10.1128/jb.176.6.1793-1795.1994
PMID:8132479
原文链接:https://pmc.ncbi.nlm.nih.gov/articles/PMC205273/
Abstract

Escherichia coli protease I is assayed as an esterase active with certain synthetic model chymotrypsin substrates. However, the gene encoding protease I has the same DNA sequence and genomic location as tesA, a gene that encodes E. coli thioesterase I. We report that both hydrolase activities utilize the same active site and demonstrate that the protein functions as a thioesterase in vivo.

摘要

大肠杆菌蛋白酶I作为一种酯酶，对某些合成的胰凝乳蛋白酶底物具有活性。然而，编码蛋白酶I的基因与tesA具有相同的DNA序列和基因组位置，tesA是一个编码大肠杆菌硫酯酶I的基因。我们报告这两种水解酶活性利用相同的活性位点，并证明该蛋白在体内作为硫酯酶发挥作用。

https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ffa/205273/37775618bea1/jbacter00024-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ffa/205273/37775618bea1/jbacter00024-0252-a.jpg
https://cdn.ncbi.nlm.nih.gov/pmc/blobs/5ffa/205273/37775618bea1/jbacter00024-0252-a.jpg

相似文献

1
"Protease I" of Escherichia coli functions as a thioesterase in vivo.大肠杆菌的“蛋白酶I”在体内发挥硫酯酶的作用。
J Bacteriol. 1994 Mar;176(6):1793-5. doi: 10.1128/jb.176.6.1793-1795.1994.
2
Escherichia coli thioesterase I, molecular cloning and sequencing of the structural gene and identification as a periplasmic enzyme.大肠杆菌硫酯酶I，结构基因的分子克隆与测序以及作为周质酶的鉴定
J Biol Chem. 1993 May 5;268(13):9238-45.
3
Cloning, sequencing, and characterization of Escherichia coli thioesterase II.大肠杆菌硫酯酶II的克隆、测序及特性分析。
J Biol Chem. 1991 Jun 15;266(17):11044-50.
4
Genetic and biochemical characterization of an Escherichia coli K-12 mutant deficient in acyl-coenzyme A thioesterase II.缺乏酰基辅酶A硫酯酶II的大肠杆菌K-12突变体的遗传和生化特性
J Bacteriol. 1986 Mar;165(3):911-7. doi: 10.1128/jb.165.3.911-917.1986.
5
The Escherichia coli pldC gene encoding lysophospholipase L(1) is identical to the apeA and tesA genes encoding protease I and thioesterase I, respectively.编码溶血磷脂酶L(1)的大肠杆菌pldC基因分别与编码蛋白酶I和硫酯酶I的apeA和tesA基因相同。
J Biochem. 1999 Aug;126(2):445-8. doi: 10.1093/oxfordjournals.jbchem.a022470.
6
Functional role of a non-active site residue Trp(23) on the enzyme activity of Escherichia coli thioesterase I/protease I/lysophospholipase L(1).非活性位点残基色氨酸（23）对大肠杆菌硫酯酶I/蛋白酶I/溶血磷脂酶L（1）酶活性的功能作用
Biochim Biophys Acta. 2009 Oct;1794(10):1467-73. doi: 10.1016/j.bbapap.2009.06.008. Epub 2009 Jun 18.
7
Identification of a hotdog fold thioesterase involved in the biosynthesis of menaquinone in Escherichia coli.鉴定大肠杆菌中参与menaquinone 生物合成的热狗折叠硫酯酶。
J Bacteriol. 2013 Jun;195(12):2768-75. doi: 10.1128/JB.00141-13. Epub 2013 Apr 5.
8
A novel paradigm of fatty acid beta-oxidation exemplified by the thioesterase-dependent partial degradation of conjugated linoleic acid that fully supports growth of Escherichia coli.一种以硫酯酶依赖性共轭亚油酸部分降解为代表的脂肪酸β-氧化新范式，该范式完全支持大肠杆菌的生长。
Biochemistry. 2008 Sep 9;47(36):9618-26. doi: 10.1021/bi801074e. Epub 2008 Aug 15.
9
Backbone dynamics of Escherichia coli thioesterase/protease I: evidence of a flexible active-site environment for a serine protease.大肠杆菌硫酯酶/蛋白酶I的主链动力学：丝氨酸蛋白酶活性位点环境灵活的证据。
J Mol Biol. 2001 Apr 6;307(4):1075-90. doi: 10.1006/jmbi.2001.4539.
10
[Improvement of fatty acid ethyl ester production by optimizing thioesterase expression].通过优化硫酯酶表达提高脂肪酸乙酯产量
Sheng Wu Gong Cheng Xue Bao. 2013 Nov;29(11):1681-6.

引用本文的文献

1
Elucidation of transient protein-protein interactions within carrier protein-dependent biosynthesis.阐明载体蛋白依赖性生物合成过程中的瞬时蛋白质-蛋白质相互作用。
Commun Biol. 2021 Mar 16;4(1):340. doi: 10.1038/s42003-021-01838-3.
2
Investigating host-bacterial interactions among enteric pathogens.研究肠道病原体中宿主-细菌的相互作用。
BMC Genomics. 2019 Dec 27;20(1):1022. doi: 10.1186/s12864-019-6398-2.
3
Multifunctionality and diversity of GDSL esterase/lipase gene family in rice (Oryza sativa L. japonica) genome: new insights from bioinformatics analysis.

本文引用的文献

1
A rapid method of total lipid extraction and purification.一种快速的总脂质提取与纯化方法。
Can J Biochem Physiol. 1959 Aug;37(8):911-7. doi: 10.1139/o59-099.
2
Three-dimensional structure of the complex between acyl-coenzyme A binding protein and palmitoyl-coenzyme A.酰基辅酶A结合蛋白与棕榈酰辅酶A复合物的三维结构
J Mol Biol. 1993 Apr 20;230(4):1260-77. doi: 10.1006/jmbi.1993.1240.
3
Molecular cloning, sequencing, and mapping of the gene encoding protease I and characterization of proteinase and proteinase-defective Escherichia coli mutants.
水稻（Oryza sativa L. japonica）基因组中 GDSL 酯酶/脂肪酶基因家族的多功能性和多样性：生物信息学分析的新见解。
BMC Genomics. 2012 Jul 15;13:309. doi: 10.1186/1471-2164-13-309.
4
The Burkholderia cenocepacia BDSF quorum sensing fatty acid is synthesized by a bifunctional crotonase homologue having both dehydratase and thioesterase activities.伯克霍尔德氏菌 BDSF 群体感应脂肪酸由具有脱水酶和硫酯酶活性的双功能丙二酰辅酶 A 同源物合成。
Mol Microbiol. 2012 Feb;83(4):840-55. doi: 10.1111/j.1365-2958.2012.07968.x. Epub 2012 Jan 29.
5
Functional role of catalytic triad and oxyanion hole-forming residues on enzyme activity of Escherichia coli thioesterase I/protease I/phospholipase L1.催化三联体和氧阴离子空穴形成残基对大肠杆菌硫酯酶I/蛋白酶I/磷脂酶L1酶活性的功能作用
Biochem J. 2006 Jul 1;397(1):69-76. doi: 10.1042/BJ20051645.
6
Inhibition of Escherichia coli acetyl coenzyme A carboxylase by acyl-acyl carrier protein.酰基-酰基载体蛋白对大肠杆菌乙酰辅酶A羧化酶的抑制作用。
J Bacteriol. 2001 Feb;183(4):1499-503. doi: 10.1128/JB.183.4.1499-1503.2001.
7
Linkage map of Escherichia coli K-12, edition 10: the traditional map.大肠杆菌K-12连锁图谱，第10版：传统图谱。
Microbiol Mol Biol Rev. 1998 Sep;62(3):814-984. doi: 10.1128/MMBR.62.3.814-984.1998.
8
Multinuclear NMR resonance assignments and the secondary structure of Escherichia coli thioesterase/protease I: a member of a new subclass of lipolytic enzymes.
J Biomol NMR. 1998 May;11(4):363-80. doi: 10.1023/a:1008226515482.
9
The apeE gene of Salmonella typhimurium encodes an outer membrane esterase not present in Escherichia coli.鼠伤寒沙门氏菌的apeE基因编码一种大肠杆菌中不存在的外膜酯酶。
J Bacteriol. 1998 Jul;180(14):3517-21. doi: 10.1128/JB.180.14.3517-3521.1998.
10
In vivo evidence that acyl coenzyme A regulates DNA binding by the Escherichia coli FadR global transcription factor.体内证据表明，酰基辅酶A可调节大肠杆菌全局转录因子FadR与DNA的结合。
J Bacteriol. 1997 Mar;179(5):1819-23. doi: 10.1128/jb.179.5.1819-1823.1997.
蛋白酶I编码基因的分子克隆、测序与定位以及蛋白酶和蛋白酶缺陷型大肠杆菌突变体的特性分析
J Bacteriol. 1993 Feb;175(4):1032-7. doi: 10.1128/jb.175.4.1032-1037.1993.
4
Escherichia coli thioesterase I, molecular cloning and sequencing of the structural gene and identification as a periplasmic enzyme.大肠杆菌硫酯酶I，结构基因的分子克隆与测序以及作为周质酶的鉴定
J Biol Chem. 1993 May 5;268(13):9238-45.
5
Characterization of ligand binding to acyl-CoA-binding protein.配体与酰基辅酶A结合蛋白的结合特性分析。
Biochem J. 1993 Mar 1;290 ( Pt 2)(Pt 2):321-6. doi: 10.1042/bj2900321.
6
Isolation and some propeties of a proteolytic enzyme from Escherichia coli (protease I).大肠杆菌中一种蛋白水解酶（蛋白酶I）的分离及某些特性
Eur J Biochem. 1971 Dec 10;23(3):435-42. doi: 10.1111/j.1432-1033.1971.tb01638.x.
7
Purification and properties of a palmityl thioesterase II from Escherichia coli.来自大肠杆菌的棕榈酰硫酯酶II的纯化及特性
J Biol Chem. 1970 Jun;245(12):3122-8.
8
Studies on the mechanism of fatty acid synthesis. XIX. Preparation and general properties of palmityl thioesterase.脂肪酸合成机制的研究。第十九部分。棕榈酰硫酯酶的制备及一般性质。
J Biol Chem. 1968 Jun 10;243(11):2955-62.
9
Beta-hydroxydecanoyl thioester dehydrase. I. Purification and properties.β-羟基癸酰硫酯脱水酶。I. 纯化及性质
J Biol Chem. 1967 Oct 10;242(19):4418-31.
10
A molecular view of fatty acid catabolism in Escherichia coli.大肠杆菌中脂肪酸分解代谢的分子视角。
Microbiol Rev. 1986 Jun;50(2):179-92. doi: 10.1128/mr.50.2.179-192.1986.