Sawada N, Kojima T, Obata H, Isomura H, Atsumi S, Sawaki M, Tsuzuki N, Tobioka H, Kokai Y, Satoh M, Mori M
Department of Pathology, Cancer Research Institute, Sapporo Medical University School of Medicine, Chuo-ku.
Jpn J Cancer Res. 1996 Nov;87(11):1102-5. doi: 10.1111/j.1349-7006.1996.tb03117.x.
It is reported that hepatocytes isolated from LEC rats with chronic liver injury show reduced growth activity in primary culture. To elucidate the molecular basis of this phenomenon, we examined expression of p21(waf-1/ciP-1) and p27, cyclin-dependent kinase inhibitors, by northern blot analysis. The expression of p21(waf-1/cip-1 ) in the LEC rat liver was 3-fold higher than that of age-matched SD rat liver, while there was no significant difference in p27 expression level. Western blot analysis also revealed a significant increase in p21(waf-1/cip-1) in the nuclear matrix fraction of the LEC rat liver. Immunohistochemically, p21(waf-1/cip-1) was detected in the nuclei of normal LEC rat hepatocytes, but not in those of hepatocellular carcinoma cells, suggesting selective growth of neoplastic hepatocytes.
据报道，从患有慢性肝损伤的LEC大鼠分离出的肝细胞在原代培养中显示出生长活性降低。为了阐明这种现象的分子基础，我们通过Northern印迹分析检测了细胞周期蛋白依赖性激酶抑制剂p21（waf-1/ciP-1）和p27的表达。LEC大鼠肝脏中p21（waf-1/cip-1）的表达比年龄匹配的SD大鼠肝脏高3倍，而p27表达水平没有显著差异。蛋白质免疫印迹分析还显示LEC大鼠肝脏核基质部分中p21（waf-1/cip-1）显著增加。免疫组织化学显示，在正常LEC大鼠肝细胞的细胞核中检测到p21（waf-1/cip-1），但在肝癌细胞的细胞核中未检测到，这表明肿瘤性肝细胞的选择性生长。
