Reisen W K, Hardy J L, Chiles R E, Kramer L D, Martinez V M, Presser S B
Arbovirus Research Program, Division of Epidemiology and Public Health Biology, School of Public Health, University of California, Berkeley 94720, USA.
J Am Mosq Control Assoc. 1996 Dec;12(4):679-87.
During 1994-95, totals of 17,656 adult females and 111,104 adults reared from field-collected immatures comprising 19 species in 4 genera of mosquitoes were collected from Morro Bay estuary and surrounding environs in San Luis Obispo County, California. Aedes dorsalis was the dominant summer mosquito, whereas Aedes squamiger and Ae. washinoi were abundant during winter and early spring. Host-seeking Culex tarsalis were collected infrequently, even though immatures were collected frequently from freshwater surface pools. Overall, 13,561 adults (386 pools) and 91,547 adults reared from field-collected immatures (3,027 pools) were tested for arboviruses by plaque assay in Vero cell culture. Morro Bay virus, a member of the California serogroup, was isolated from 4 pools of Ae. squamiger reared from field-collected immatures (minimum field infection rate-1.07 per 1,000), verifying the maintenance of this virus by vertical transmission. All remaining pools were negative. Three flocks of 10 sentinel chickens and one group of 5 sentinel rabbits were bled biweekly and tested for arbovirus antibodies with negative results. Neither horizontal nor vertical transmission of western equine encephalomyelitis virus was detected.
1994 - 1995年期间，从加利福尼亚州圣路易斯奥比斯波县的莫罗湾河口及周边地区收集了17656只成年雌性蚊子和111104只从野外采集的未成熟蚊子饲养而来的成年蚊子，这些蚊子包括4个属的19个物种。背点伊蚊是夏季的优势蚊子，而鳞斑伊蚊和瓦氏伊蚊在冬季和早春数量众多。尽管经常从淡水表层水池中采集到未成熟的尖音库蚊，但很少采集到寻找宿主的尖音库蚊。总体而言，通过在Vero细胞培养中进行蚀斑测定，对13561只成蚊（386个水池）和91547只从野外采集的未成熟蚊子饲养而来的成蚊（3027个水池）进行了虫媒病毒检测。从4个由野外采集的未成熟蚊子饲养而来的鳞斑伊蚊水池中分离出了加利福尼亚血清群的成员莫罗湾病毒（最低野外感染率为每1000只1.07只），证实了该病毒通过垂直传播得以维持。其余所有水池检测结果均为阴性。对3组每组10只哨兵鸡和1组5只哨兵兔每两周采血一次，并检测虫媒病毒抗体，结果均为阴性。未检测到西部马脑炎病毒的水平传播和垂直传播。
