Tsurumi T
Laboratory of Virology, Nagoya University School of Medicine.
Nihon Rinsho. 1997 Feb;55(2):321-7.
Epstein-Barr virus(EBV) is a human herpesvirus with a linear double stranded DNA, 172 kbp in length. The EBV has both a latent state and a lytic replicative cycle. Latency replication is dependent on cellular DNA replication machinery except for a single EBV-encoded protein, EBNA1. The EBV genome is maintained as a circular plasmid molecule and ori P, the latent phase replication origin of EBV, mediates this type of replication. The immediate-early BZLF1 protein disrupts viral latency through transactivation of early EBV genes. After induction of the lytic phase of viral replication, the EBV genome is amplified 100- to 1000-fold. The replication product is a head to tail concatemer which is presumed to be synthesized via a rolling circle mechanism initiated from the lytic phase replication origin, ori Lyt. EBV encodes seven viral genes that are essential for ori Lyt-dependent DNA replication. A number of features of EBV DNA replication make it a unique model system for the study of eukaryotic DNA replication. Here, the molecular mechanism of the lytic phase of the EBV DNA replication is discussed.
爱泼斯坦-巴尔病毒（EBV）是一种人类疱疹病毒，其基因组为线性双链DNA，长度为172千碱基对。EBV具有潜伏状态和裂解复制周期。除了单个EBV编码蛋白EBNA1外，潜伏性复制依赖于细胞DNA复制机制。EBV基因组以环状质粒分子形式维持，EBV的潜伏阶段复制起点ori P介导这种类型的复制。立即早期蛋白BZLF1通过反式激活早期EBV基因来破坏病毒潜伏状态。在诱导病毒复制的裂解阶段后，EBV基因组被扩增100至1000倍。复制产物是头尾串联体，推测是通过从裂解阶段复制起点ori Lyt起始的滚环机制合成的。EBV编码七个对ori Lyt依赖性DNA复制至关重要的病毒基因。EBV DNA复制的许多特征使其成为研究真核生物DNA复制的独特模型系统。在此，将讨论EBV DNA复制裂解阶段的分子机制。
