Mattia E, Ceridono M, Chichiarelli S, D'Erme M
Faculty of Medicine and Surgery, University of Roma "La Sapienza,", Rome, Italy.
Virology. 1999 Sep 15;262(1):9-17. doi: 10.1006/viro.1999.9854.
Eukaryotic DNA is organized into domains or loops generated by the attachment of chromatin fibers to the nuclear matrix via specific regions called scaffold or matrix attachment regions. The role of these regions in DNA replication is currently under investigation since they have been found in close association with origins of replication. Also, viral DNA sequences, containing the origins of replication, have been found attached to the nuclear matrix. To investigate the functional role of this binding we have studied, in Raji cells, the interaction between Epstein-Barr virus (EBV) origins of replication and the nuclear matrix in relation to the viral cycle of infection. We report here that both the latent (ori P) and the lytic (ori Lyt) EBV origins of replication are attached to the nuclear matrix, the first during the latent cycle of infection and the second after induction of the lytic cycle. These findings suggest that the binding of the origins of replication with the nuclear matrix modulates viral replication and expression in the two different phases of infection.
真核生物的DNA通过称为支架或基质附着区域的特定区域与核基质相连，从而被组织成结构域或环。这些区域在DNA复制中的作用目前正在研究中，因为它们已被发现与复制起点密切相关。此外，含有复制起点的病毒DNA序列也被发现附着在核基质上。为了研究这种结合的功能作用，我们在Raji细胞中研究了爱泼斯坦-巴尔病毒（EBV）复制起点与核基质之间的相互作用与病毒感染周期的关系。我们在此报告，EBV的潜伏性（ori P）和裂解性（ori Lyt）复制起点均附着于核基质，前者在潜伏感染周期中，后者在裂解周期诱导后。这些发现表明，复制起点与核基质的结合在感染的两个不同阶段调节病毒的复制和表达。
