Wu L, Brucker M, Gruskin E, Roth S I, Mustoe T A
Division of Plastic Surgery, Department of Surgery, Northwestern University Medical School, Chicago, Ill., USA.
Plast Reconstr Surg. 1997 Mar;99(3):815-22; discussion 823-4. doi: 10.1097/00006534-199703000-00030.
Platelet-derived growth factor BB (PDGF-BB) plays a central role in wound healing. Platelet-derived growth factor has been shown to accelerate healing in preclinical and clinical studies, but its wound-healing effects in older animals have not been examined. An ischemic incisional model in young female (5 months) and aged male (60 months) rabbits was used to determine the influence of platelet-derived growth factor on day 14 postwounding with reduced blood supply. Wounds in aged ischemic animals were severely impaired in breaking strength compared with their nonischemic control wounds and wounds in young animals (p < 0.001). Topical platelet-derived growth factor (10 microg per wound) partially reversed the reduction in breaking strength in aged ischemic animals but was ineffective in young animals. Histologic studies showed that new granulation tissue deposition and mononuclear cell infiltration was dramatically lower in ischemic wounds of aged animals compared with their control wounds as well as wounds from young animals under both nonischemic and ischemic conditions. Platelet-derived growth factor partially reversed this deficit in old ischemic wounds but not in young ischemic wounds. Epithelial growth was reduced in wounds from aged animals compared with wounds from young animals after 14 days of healing. Platelet-derived growth factor treatment increased ischemic wound epithelial growth in aged ischemic animals about threefold compared with paired controls. In conclusion, wound healing in aged rabbits was severely impaired by ischemia, and a single topical platelet-derived growth factor treatment partially reversed this deficit.
血小板衍生生长因子BB（PDGF - BB）在伤口愈合中起核心作用。在临床前和临床研究中，血小板衍生生长因子已被证明可加速愈合，但尚未研究其在老年动物中的伤口愈合效果。采用年轻雌性（5个月）和老年雄性（60个月）兔的缺血切口模型，以确定血小板衍生生长因子对伤后14天血供减少情况下的影响。与非缺血对照伤口及年轻动物的伤口相比，老年缺血动物伤口的抗张强度严重受损（p < 0.001）。局部应用血小板衍生生长因子（每伤口10微克）部分逆转了老年缺血动物抗张强度的降低，但对年轻动物无效。组织学研究表明，与对照伤口以及非缺血和缺血条件下年轻动物的伤口相比，老年动物缺血伤口中的新肉芽组织沉积和单核细胞浸润显著减少。血小板衍生生长因子部分逆转了老年缺血伤口中的这一缺陷，但未逆转年轻缺血伤口中的缺陷。愈合14天后，与年轻动物的伤口相比，老年动物伤口的上皮生长减少。与配对对照相比，血小板衍生生长因子治疗使老年缺血动物缺血伤口的上皮生长增加了约三倍。总之，缺血严重损害了老年兔的伤口愈合，单次局部应用血小板衍生生长因子治疗可部分逆转这一缺陷。
