Uhl Eberhard, Rösken Frank, Sirsjö Allan, Messmer Konrad
Department of Neurosurgery, Ludwig-Maximilians-University, Grosshadern University Hospital, Marchioninistrasse 15, D-81377 Munich, Germany.
Wound Repair Regen. 2003 Sep-Oct;11(5):361-7. doi: 10.1046/j.1524-475x.2003.11508.x.
The aim of the current study was to evaluate the influence of platelet-derived growth factor (PDGF) on skin microcirculation during normal and impaired wound healing. Secondary healing wounds were created on the ears of hairless mice and treated once with 3 microg of PDGF-BB immediately after wound creation. Intravital fluorescence microscopy was used to quantify reepithelialization, revascularization, vessel diameters, vascular permeability, and leukocyte-endothelium interactions up to 24 days after wound creation. Microvascular perfusion was assessed by laser Doppler flowmetry. Wound healing was studied in normal (n = 15) and ischemic skin tissue (n = 15) as well as in mice (n = 17) rendered hyperglycemic by an intravenous injection of streptozotocin 7 days prior to wound creation. Treatment with PDGF accelerated reepithelialization and reduced the time for complete wound closure in ischemic skin from 14.9 +/- 2.5 (control) to 12.3 +/- 1.8 days (p < 0.03), and in hyperglycemic animals from 15.0 +/- 2.4 (control) to 12.0 +/- 3.0 days (p < 0.04). Revascularization of these wounds was also significantly enhanced after PDFG application. No other parameters were influenced by the treatment. Normal wound healing was not affected. This study confirms the positive influence of PDGF on wound healing under pathophysiological conditions. The effects in this model seem to be primarily due to the mitogenic potency of PDGF on keratinocytes and endothelial cells. A significant effect on leukocyte activation during the inflammatory process was not observed.
本研究的目的是评估血小板衍生生长因子（PDGF）在正常和受损伤口愈合过程中对皮肤微循环的影响。在无毛小鼠耳部制造二期愈合伤口，并在伤口形成后立即用3微克的PDGF-BB处理一次。使用活体荧光显微镜对伤口形成后长达24天的再上皮化、血管再生、血管直径、血管通透性以及白细胞与内皮细胞的相互作用进行量化。通过激光多普勒血流仪评估微血管灌注。在正常皮肤组织（n = 15）和缺血皮肤组织（n = 15）以及在伤口形成前7天通过静脉注射链脲佐菌素使血糖升高的小鼠（n = 17）中研究伤口愈合情况。用PDGF治疗可加速缺血皮肤的再上皮化，并将完全伤口闭合时间从14.9±2.5天（对照组）缩短至12.3±1.8天（p < 0.03），在高血糖动物中从15.0±2.4天（对照组）缩短至12.0±3.0天（p < 0.04）。应用PDGF后，这些伤口的血管再生也显著增强。治疗未影响其他参数。正常伤口愈合不受影响。本研究证实了PDGF在病理生理条件下对伤口愈合的积极影响。该模型中的作用似乎主要归因于PDGF对角质形成细胞和内皮细胞的促有丝分裂能力。未观察到对炎症过程中白细胞激活的显著影响。
