Florea C S, Timko M P
Department of Biology, University of Virginia, Charlottesville 22903, USA.
Gene. 1997 Feb 20;186(1):127-33. doi: 10.1016/s0378-1119(96)00695-6.
In order to better understand the regulation of cellular differentiation during haustorial development in parasitic angiosperms, we have begun to examine the structure and expression characteristics of genes encoding various components of the plant cytoskeleton in Striga asiatica L. (Kuntze). We describe here the cloning and characterization of three actin genes from Striga with significant similarity at the nucleotide level and encoding proteins having greater than 98% identity. However, the three genes (designated SAAc-1, SAAc-2 and SAAc-3) differ from each other in their organization and SAAc-3 contains an unusual exon-intron arrangement relative to genes encoding actins described in other higher plants. The significance of these observations concerning the evolutionary origins and potential roles of Striga actin genes is discussed.
为了更好地理解寄生被子植物吸器发育过程中细胞分化的调控机制，我们已开始研究亚洲独脚金（Striga asiatica L. (Kuntze)）中编码植物细胞骨架各组分的基因的结构和表达特征。在此，我们描述了从独脚金中克隆出的三个肌动蛋白基因，它们在核苷酸水平上具有显著相似性，且编码的蛋白质具有大于98%的同一性。然而，这三个基因（命名为SAAc - 1、SAAc - 2和SAAc - 3）在其结构上彼此不同，并且相对于其他高等植物中编码肌动蛋白的基因，SAAc - 3含有一种不寻常的外显子 - 内含子排列。本文讨论了这些关于独脚金肌动蛋白基因的进化起源和潜在作用的观察结果的意义。
