Wolf S J, Timko M P
Department of Biology, University of Virginia, Charlottesville 22903.
Planta. 1994;192(1):61-8. doi: 10.1007/BF00198693.
Partial cDNAs and genomic fragments representing three different actin genes of witchweed (Striga asiatica L. Kuntze) have been isolated using the polymerase chain reaction. The three genes differ in nucleotide sequence within their coding and 3' non-coding regions but encode proteins identical over the regions where sequence is available. Southern blot hybridization analysis of total genomic DNA showed that the three actin genes belong to a multigene family. Expression studies using gene-specific probes showed that transcripts of each of the three Striga actin genes were present at comparable levels in roots, shoots, and 24-h-induced haustoria. The relative abundance of the three transcripts within roots or shoots, however, differed appreciably. Such differences in transcript accumulation within organs indicate that differences might exist in their cell- or tissue-specific mode of regulation.
利用聚合酶链反应已分离出代表独脚金（Striga asiatica L. Kuntze）三种不同肌动蛋白基因的部分cDNA和基因组片段。这三个基因在其编码区和3'非编码区内的核苷酸序列不同，但在可获得序列的区域编码相同的蛋白质。对总基因组DNA的Southern印迹杂交分析表明，这三个肌动蛋白基因属于一个多基因家族。使用基因特异性探针的表达研究表明，独脚金的这三个肌动蛋白基因的转录本在根、茎和24小时诱导的吸器中以相当的水平存在。然而，这三种转录本在根或茎中的相对丰度有明显差异。器官内转录本积累的这种差异表明，它们在细胞或组织特异性调控模式上可能存在差异。
