van Loosdrecht M C, Smolders G J, Kuba T, Heijnen J J
Dept. Biochemical Engineering, Kluyver Laboratory for Biotechnology, Delft, The Netherlands.
Antonie Van Leeuwenhoek. 1997 Feb;71(1-2):109-16. doi: 10.1023/a:1000150523030.
The removal of phosphorus from wastewater is already widely applied. In many cases use is made of micro organisms capable of accumulating phosphorus as polyphosphate inside the cell. The main characteristic providing the competitive advantage to these polyphosphate accumulating bacteria is the capability to use polyphosphate, in the absence of external electron acceptors, as energy source for the uptake and storage of acetic acid in the form of polyhydroxybutyrate (PHB). The reduction equivalents for the formation of PHB are derived from the conversion of glycogen to PHB. Despite the widespread use and study of enhanced biological phosphorus removal no pure culture, having the above mentioned characteristics, has been isolated yet. All ecophysiological studies on these type of cultures have therefore been performed by enrichment cultures. This paper reviews the research on these type of organisms, and shows that it is possible to understand a complex microbial process on a metabolic level, both stoichiometrically and kinetically, without the availability of a pure culture.
从废水中去除磷已经得到广泛应用。在许多情况下，会利用能够在细胞内将磷以多聚磷酸盐形式积累的微生物。这些聚磷菌具有竞争优势的主要特性是，在没有外部电子受体的情况下，能够利用多聚磷酸盐作为能量来源，将乙酸以聚羟基丁酸酯（PHB）的形式摄取和储存。用于形成PHB的还原当量来自糖原向PHB的转化。尽管强化生物除磷已得到广泛应用和研究，但尚未分离出具有上述特性的纯培养物。因此，所有关于这类培养物的生态生理学研究都是通过富集培养进行的。本文综述了对这类生物的研究，并表明在没有纯培养物的情况下，从代谢水平上，无论是化学计量还是动力学方面，理解一个复杂的微生物过程是可能的。
