藻酸盐凝胶作为研究紫外线A辐射对人皮肤成纤维细胞影响的培养模型的局限性。

Limitations of alginate gels as a culture model for the study of the effects of UVA radiation on human dermal fibroblasts.

作者信息

Le Tallec L P, Korwin-Zmijowska C, Adolphe M

机构信息

Laboratoire de Pharmacologie Cellulaire de l'Ecole Pratique des Hautes Etudes, Paris, France.

出版信息

Cell Biol Toxicol. 1997 Feb;13(2):95-102. doi: 10.1023/b:cbto.0000010394.58263.2c.

DOI:10.1023/b:cbto.0000010394.58263.2c

PMID:9049099

Abstract

Several studies were undertaken to develop three-dimensional (3-D) cell culture models that allow conditions closer to the in vivo situation. To this end, alginate gels were tested as a 3-D cell culture model that might be useful in the study of the effects of UVA on human dermal fibroblasts. Cell culture in alginate gels and the irradiation conditions were optimized. Results showed that optimized cultures in alginate gels experienced considerable cell death on UVA irradiation compared to the classical monolayer cell culture. Viability tests (cell counting and neutral red assay) were performed to show that only UVA-irradiated alginate gels were responsible for this cytotoxicity. The implication of oxygen species in the phototoxicity induced by ultraviolet light has already been described; for this reason we investigated whether oxygen species were involved in the cytotoxicity induced by alginate upon UVA irradiation. It appeared that superoxide anion is not implicated.

摘要

开展了多项研究以开发三维（3-D）细胞培养模型，使其条件更接近体内情况。为此，测试了藻酸盐凝胶作为一种3-D细胞培养模型，其可能有助于研究紫外线A（UVA）对人皮肤成纤维细胞的影响。优化了藻酸盐凝胶中的细胞培养和照射条件。结果表明，与传统的单层细胞培养相比，藻酸盐凝胶中的优化培养在UVA照射下经历了相当多的细胞死亡。进行了活力测试（细胞计数和中性红试验）以表明只有UVA照射的藻酸盐凝胶导致了这种细胞毒性。紫外线诱导的光毒性中活性氧的作用已经被描述；因此，我们研究了活性氧是否参与了UVA照射后藻酸盐诱导的细胞毒性。结果显示超氧阴离子与此无关。