Almazán F, Murguía J R, Rodríguez J M, de la Vega I, Viñuela E
Centro de Biología Molecular Severo Ochoa (CSIC-UAM), Facultad de Ciencias, Universidad Autónoma, Cantoblanco, Madrid, Spain.
J Gen Virol. 1995 Apr;76 ( Pt 4):729-40. doi: 10.1099/0022-1317-76-4-729.
A group of cross-hybridizing DNA segments contained within the EcoRI restriction fragments U', X and J of a Vero cell-adapted strain (BA71V) of African swine fever virus (ASFV) were mapped and sequenced. Analysis of the nucleotide sequence revealed the presence of a set of long internal repeated sequences composed of five types of tandemly repeat units of about 200 bp. These tandem repeats contain a G-rich core of 10-14 nucleotides surrounded by regions with a high A + T content distributed in oligo(dA).oligo(dT) tracts. Next to the repeated sequences we detected two related open reading frames that are members of a new multigene family (multigene family 300). Comparison of DNA sequences from several virus isolates indicated that this region undergoes frequent rearrangements leading to either duplications or deletions of the repeat units. These ASFV repeated sequences share similarities with chromosomal alpha satellite DNA, the scaffold-associated region and satellite III of Drosophila. Similar tandemly repeated sequences have not been described in other viruses.
对非洲猪瘟病毒（ASFV）的Vero细胞适应株（BA71V）的EcoRI限制性片段U'、X和J中包含的一组交叉杂交DNA片段进行了图谱绘制和测序。核苷酸序列分析显示存在一组由五种约200 bp的串联重复单元组成的长内部重复序列。这些串联重复序列包含一个10 - 14个核苷酸的富含G的核心，周围是A + T含量高的区域，分布在寡聚（dA）·寡聚（dT）片段中。在重复序列旁边，我们检测到两个相关的开放阅读框，它们是一个新的多基因家族（多基因家族300）的成员。来自几种病毒分离株的DNA序列比较表明，该区域经常发生重排，导致重复单元的复制或缺失。这些ASFV重复序列与染色体α卫星DNA、果蝇的支架相关区域和卫星III具有相似性。其他病毒中尚未描述过类似的串联重复序列。
