Meireles M, Costa J V
Laboratory of Virology II, Gulbenkian Institute of Science, Oeiras, Portugal.
Virology. 1994 Aug 15;203(1):193-6. doi: 10.1006/viro.1994.1474.
The 4-kb terminal BclI fragment of African swine fever virus (ASFV) DNA, including the inverted terminal repetition (ITR), was cloned and sequenced. The sequence showed that the 2.1-kb ITR is composed of a 301-nucleotide-long unique sequence immediately adjacent to the terminal hairpin loop, a set of 38 tandem direct repeats of a 34-nucleotide degenerate sequence, another unique intervening sequence with no remarkable features, and a set of 5 tandem repeats of a 27-nucleotide unit unrelated in sequence to the 34-nucleotide repeat unit. Several short repeats were found scattered along the most distal unique sequence. The proximal half of the terminal fragment possesses one complete and one interrupted copy of genes belonging to multigene family 360.
对非洲猪瘟病毒（ASFV）DNA的4kb末端BclI片段进行了克隆和测序，该片段包含反向末端重复序列（ITR）。序列分析表明，2.1kb的ITR由紧邻末端发夹环的一段301个核苷酸长的独特序列、一组38个串联的34个核苷酸简并序列的直接重复序列、另一段无明显特征的独特间隔序列以及一组5个串联的27个核苷酸单元的重复序列组成，该27个核苷酸单元的序列与34个核苷酸重复单元无关。沿着最远端的独特序列发现了几个短重复序列。末端片段的近端一半拥有属于多基因家族360的一个完整基因拷贝和一个中断的基因拷贝。
