Loudes A M, Ritzenthaler C, Pinck M, Serghini M A, Pinck L
Institut de Biologie Moléculaire des Plantes du CNRS et Université Louis Pasteur, Strasbourg, France.
J Gen Virol. 1995 Apr;76 ( Pt 4):899-906. doi: 10.1099/0022-1317-76-4-899.
Arabis mosaic virus (ArMV) is a nepovirus that is serologically distantly related to grapevine fanleaf virus (GFLV). Both ArMV and GFLV induce grapevine degeneration disease. Several ArMV isolates, unlike isolates of GFLV, produce upon in vitro translation of RNA2 a polyprotein (P2) that forms a double band in polyacrylamide-SDS gels. Cloning of full-length copies of RNA2 of an ArMV isolate from grapevine (ArMV-S) revealed that this isolate contained two RNA2s of different length, called RNA2-U and RNA2-L. The two species were not readily separated by electrophoresis of the virion RNA under denaturing gel electrophoresis conditions but could be distinguished by analysis of primer extension and in vitro translation products. The size difference of the two RNA2s is due mostly if not exclusively to differences in their coding regions. The 124 kDa RNA2-U-encoded polyprotein P2' and the 119 kDa RNA2-L-encoded polyprotein P2", which co-migrate, respectively, with the upper and lower polyprotein bands produced by RNA2 of ArMV-S, were more than 95% identical except in their N-terminal domains. In vitro maturation experiments and sequence comparisons indicate that the N-terminal products of P2' and P2" have a molecular mass of 31 kDa and 26 kDa. The genomic organization proposed is similar to that of GFLV RNA2.
南芥菜花叶病毒（ArMV）是一种线虫传多面体病毒，在血清学上与葡萄扇叶病毒（GFLV）亲缘关系较远。ArMV和GFLV均可引发葡萄退化病。与GFLV的分离株不同，一些ArMV分离株在RNA2的体外翻译过程中产生一种多聚蛋白（P2），该多聚蛋白在聚丙烯酰胺-SDS凝胶中形成一条双带。对一株来自葡萄的ArMV分离株（ArMV-S）的RNA2全长拷贝进行克隆后发现，该分离株含有两种长度不同的RNA2，分别称为RNA2-U和RNA2-L。在变性凝胶电泳条件下，这两种RNA2在病毒粒子RNA电泳中不易分离，但可通过引物延伸分析和体外翻译产物进行区分。这两种RNA2的大小差异主要（如果不是唯一的话）归因于它们编码区的差异。由RNA2-U编码的124 kDa多聚蛋白P2'和由RNA2-L编码的119 kDa多聚蛋白P2"，分别与ArMV-S的RNA2产生的上、下多聚蛋白带共迁移，除了N端结构域外，它们的同源性超过95%。体外成熟实验和序列比较表明，P2'和P2"的N端产物分子量分别为31 kDa和26 kDa。所提出的基因组结构与GFLV RNA2的相似。
