Clerehugh V, Seymour G J, Bird P S, Cullinan M, Drucker D B, Worthington H V
Department of Periodontology, Leeds Dental Institute, UK.
J Clin Periodontol. 1997 Jan;24(1):57-64. doi: 10.1111/j.1600-051x.1997.tb01185.x.
The aim of the study was to compare the occurrence and levels of A. actinomycetemcomitans, P. gingivalis, and P. intermedia in the subgingival plaque from sites with and without early periodontitis in adolescents using an ELISA. 47, 15- to 16-year-old adolescents (39 Indo-Pakistani, 8 white Caucasian) were examined for clinical attachment level, probing depth, supragingival plaque, subgingival calculus and bleeding on probing on the mesio-buccal and disto-buccal aspects of the 1st molars and the incisors. Based on the clinical data, 2 sites per subject were selected for subgingival plaque sampling 3 weeks later: in 32 subjects with loss of attachment > or = 1 mm, a diseased site (D) and a healthy comparison control site (C) were sampled; in 15 subjects in whom loss of attachment had not yet developed, 1 of the upper molar sites was selected, called the at-risk site (R), together with a C site. The presence and levels of A. actinomycetemcomitans, P. gingivalis, and P. intermedia were determined using an ELISA. The loss of attachment subgroup had significantly more pockets > or = 4 mm, subgingival calculus and bleeding on probing (p < 0.05). Significantly more of the D than C sites had P. gingivalis both at detectable and at measurable levels (p < 0.05). In subjects who had no loss in clinical attachment levels, fewer sampled sites harboured any of the suspected periodontopathogens investigated, and no significant differences were found between the R or C sites (p > 0.05). Although there was a significantly higher prevalence and extent of loss of attachment > or = 1 mm in the Indo-Pakistani subjects compared with the Caucasians (p < 0.05), no differences could be identified in the distribution of the bacteria. It is concluded that monitoring of the subgingival plaque may be useful in studies of early periodontitis in adolescents, and the role of P. gingivalis needs to be elucidated in prospective longitudinal investigations.
本研究的目的是使用酶联免疫吸附测定法（ELISA）比较青少年中存在和不存在早期牙周炎部位的龈下菌斑中伴放线放线杆菌、牙龈卟啉单胞菌和中间普氏菌的发生率及水平。对47名15至16岁的青少年（39名印度 - 巴基斯坦裔、8名白种人）的第一磨牙和切牙的近中颊侧和远中颊侧进行临床附着水平、探诊深度、龈上菌斑、龈下牙石及探诊出血情况检查。根据临床数据，3周后为每位受试者选择2个部位进行龈下菌斑采样：在32名附着丧失≥1mm的受试者中，对一个患病部位（D）和一个健康对照部位（C）进行采样；在15名尚未出现附着丧失的受试者中，选择一个上颌磨牙部位，称为高危部位（R），以及一个C部位。使用ELISA测定伴放线放线杆菌、牙龈卟啉单胞菌和中间普氏菌的存在及水平。附着丧失亚组中深度≥4mm的牙周袋、龈下牙石及探诊出血明显更多（p<0.05）。在可检测和可测量水平上，D部位牙龈卟啉单胞菌的数量明显多于C部位（p<0.05）。在临床附着水平未丧失的受试者中，较少的采样部位含有所研究的任何可疑牙周病原体，且R部位和C部位之间未发现显著差异（p>0.05）。尽管与白种人相比，印度 - 巴基斯坦裔受试者中附着丧失≥1mm的患病率和程度明显更高（p<0.05），但在细菌分布上未发现差异。结论是，监测龈下菌斑可能有助于青少年早期牙周炎的研究，牙龈卟啉单胞菌的作用需要在前瞻性纵向研究中阐明。
