López N J, Mellado J C, Leighton G X
Section of Periodontics, University of Chile, Santiago.
J Clin Periodontol. 1996 Feb;23(2):101-5. doi: 10.1111/j.1600-051x.1996.tb00541.x.
The occurrence of Actinobacillus actinomycetemcomitans, Porphyromonas gingivalis and Prevotella intermedia in subgingival plaque in 24 juvenile periodontitis patients was determined using DNA probe. 36 samples of subgingival plaque from 36 pockets having > or = 6 mm depth, > or = 3 mm of loss of attachment, and bleeding on probing and/or suppuration were taken from 18 patients with localized juvenile periodontitis (LJP, age range 12-24 years); and 12 samples from 6 patients with generalized juvenile periodontitis (GJP, age range 23-26 years). As control, an equal numbers of samples from healthy sites in the same patients were studied. P. gingivalis was found in 17 of 18 LJP patients, and in 31 of 36 diseased sites in those patients. P. intermedia was found in 15 out of the 18 LJP patients and in 28 of the 36 diseased sites. A. actinomycetemcomitans was present in 7 of the 18 LJP patients, and in 9 of the 36 diseased sites, and was not found in any GJP patients. All GJP patients had P. gingivalis (11 out of 12 diseased sites) and P. intermedia (all of the diseased sites). None of the three bacterial species was detected in healthy sites of GJP patients, and were found in healthy sites in only 2 of 18 LJP patients. The high prevalence and high levels of P. gingivalis and P. intermedia found in the LJP and GJP patients studied, suggest that there are populations affected by juvenile periodontitis in which this type of periodontitis is more associated with these species than with A. actinomycetemcomitans.
使用DNA探针测定了24例青少年牙周炎患者龈下菌斑中伴放线放线杆菌、牙龈卟啉单胞菌和中间普氏菌的检出情况。从18例局限性青少年牙周炎（LJP，年龄范围12 - 24岁）患者的36个深度≥6mm、附着丧失≥3mm且探诊出血和/或有溢脓的牙周袋中采集了36份龈下菌斑样本；从6例广泛性青少年牙周炎（GJP，年龄范围23 - 26岁）患者中采集了12份样本。作为对照，对同一患者健康部位的等量样本进行了研究。18例LJP患者中有17例检出牙龈卟啉单胞菌，这些患者的36个患病部位中有31个检出该菌。18例LJP患者中有15例检出中间普氏菌，36个患病部位中有28个检出该菌。18例LJP患者中有7例检出伴放线放线杆菌，36个患病部位中有9个检出该菌，而所有GJP患者中均未检出。所有GJP患者均有牙龈卟啉单胞菌（12个患病部位中的11个）和中间普氏菌（所有患病部位）。在GJP患者的健康部位未检测到这三种细菌中的任何一种，仅在18例LJP患者中的2例健康部位检测到。在本研究的LJP和GJP患者中发现牙龈卟啉单胞菌和中间普氏菌的高患病率及高检出水平，表明在受青少年牙周炎影响的人群中，这类牙周炎与这些菌种的关联比与伴放线放线杆菌的关联更为密切。
