Cooper D M, Swanson D L, Gebhart C J
Division of Comparative Medicine, Medical School, University of Minnesota, Minneapolis 55455, USA.
Vet Microbiol. 1997 Jan;54(1):47-62. doi: 10.1016/s0378-1135(96)01264-3.
Proliferative enteritis (PE) is an enteric disease that has been reported in a variety of animals. It is caused by an obligate intracellular bacterium identified in swine as Lawsonia intracellularis. The organism can be detected ante-mortem in swine with PE using molecular diagnostic methods. The disease can be diagnosed post-mortem in all species by gross examination of tissues and special histologic staining procedures. In this study we extracted total DNA from frozen or formalin-fixed, paraffin-embedded tissues from cases of pig, hamster, horse, deer and ostrich PE. The samples were subjected to a multiplex PCR reaction using primers specific for a swine isolate of L. intracellularis. Identical sized PCR products were detected in samples from all animals with PE and the specificity of the PCR reaction for L. intracellularis was demonstrated by Southern-blotting and hybridization using specific probes. These results suggest that the intracellular organism of PE in these species are all very closely related to the causative agent of PE in swine, L. intracellularis. In addition, this multiplex PCR assay can be used to detect the organism in frozen or archival tissues, facilitating retrospective diagnosis of PE.
增生性肠炎（PE）是一种已在多种动物中报道过的肠道疾病。它由一种专性细胞内细菌引起，在猪中该细菌被鉴定为胞内劳森菌。使用分子诊断方法可在生前检测出患有PE的猪体内的这种细菌。通过对组织进行大体检查和特殊组织学染色程序，可在死后对所有物种进行该病的诊断。在本研究中，我们从猪、仓鼠、马、鹿和鸵鸟PE病例的冷冻或福尔马林固定、石蜡包埋组织中提取了总DNA。使用针对胞内劳森菌猪分离株的特异性引物对样本进行多重PCR反应。在所有患有PE的动物样本中均检测到大小相同的PCR产物，并且通过Southern印迹法和使用特异性探针的杂交证明了PCR反应对胞内劳森菌的特异性。这些结果表明，这些物种中PE的细胞内细菌都与猪PE的病原体胞内劳森菌密切相关。此外，这种多重PCR检测方法可用于检测冷冻或存档组织中的该细菌，有助于对PE进行回顾性诊断。
