Hostacká A, Majtán V
Institute of Preventive and Clinical Medicine, Bratislava, Slovak Republic.
Microbios. 1996;87(352):141-7.
Suppression of elastase, proteinase and alginate of Pseudomonas aeruginosa after the exposure of a bacterial suspension (30 min) to supra-inhibitory concentrations (2x or 4x MIC) of norfloxacin was found. The norfloxacin at 4x MIC was more effective, and this concentration depressed elastase to 13.0%, proteinase to 14.6% and alginate to 0% of the control values. Enoxacin at 2x MIC did not affect the virulence factors studied, and at a concentration of 4x MIC only elastase was effectively reduced to 61% of the control value. Supra-subinhibitory concentrations of the quinolones tested (2x or 4x MIC + sub-MICs) severely diminished all of the activities tested compared with supra-inhibitory concentrations alone. Combinations of 4x MIC + sub-MICs were more effective in the majority of cases.
研究发现，将铜绿假单胞菌菌悬液暴露于超抑菌浓度（2倍或4倍最低抑菌浓度）的诺氟沙星30分钟后，其弹性蛋白酶、蛋白酶和藻酸盐的产生受到抑制。4倍最低抑菌浓度的诺氟沙星效果更佳，该浓度可使弹性蛋白酶降至对照值的13.0%，蛋白酶降至14.6%，藻酸盐降至0%。2倍最低抑菌浓度的依诺沙星对所研究的毒力因子无影响，在4倍最低抑菌浓度时，仅弹性蛋白酶有效降至对照值的61%。与单独的超抑菌浓度相比，所测试喹诺酮类药物的超亚抑菌浓度（2倍或4倍最低抑菌浓度+亚抑菌浓度）严重降低了所有测试活性。在大多数情况下，4倍最低抑菌浓度+亚抑菌浓度的组合效果更佳。
