Chen C T, Wang C Y, Kuo Y S, Chiang H H, Chow S N, Hsiao I Y, Chiang C P
Laser Medicine Research Center, College of Medicine, National Taiwan University, Taipei, R.O.C.
Proc Natl Sci Counc Repub China B. 1996 Oct;20(4):123-30.
For the early detection of oral neoplasia, light-induced fluorescence spectroscopy was used to measure the fluorescence emission of malignant (squamous cell carcinoma & verrucous carcinoma) and premalignant (epithelial dysplasia, hyperkeratosis & lichen planus) oral tissues as well as normal oral mucosa ex vivo to assess the ability of this technique to distinguish neoplastic from normal oral tissues. The emission spectra of histologically normal and neoplastic oral tissues were obtained under excitation wavelengths varied from 270 nm to 400 nm at 10-nm intervals. At 300-nm excitation, the most intensely fluorescent peak occurred at 330-nm and 470 nm emission. At 330-nm emission, the spectrum of the malignant oral tissue was significantly stronger than that of the normal oral mucosal tissue after area normalization. However, at 470-nm emission, the spectrum of the malignant oral tissue was significantly weaker than that of the normal oral mucosal tissue. A diagnostic algorithm based on the ratio of relative intensities of 330 nm to 470 nm emission within the +/-5 nm peak area of each sample was calculated and paired. The histogram of ratios showed that histologically neoplastic oral tissues could be distinguished from normal oral mucosal tissues using the 300 nm excitation wavelength. The average ratio of malignant or premalignant oral samples was significantly greater than that of the normal oral mucosal samples (p < 0.001). This ex vivo study indicated that fluorescence spectroscopy may be useful in differentiating malignant or premalignant oral tissue from normal oral mucosa.
为了早期检测口腔肿瘤，采用光诱导荧光光谱法对离体的恶性（鳞状细胞癌和疣状癌）、癌前（上皮发育异常、角化过度和扁平苔藓）口腔组织以及正常口腔黏膜的荧光发射进行测量，以评估该技术区分肿瘤性口腔组织和正常口腔组织的能力。在激发波长从270 nm到400 nm、间隔为10 nm的条件下，获取组织学正常和肿瘤性口腔组织的发射光谱。在300 nm激发下，最强的荧光峰出现在330 nm和470 nm发射处。在330 nm发射时，经面积归一化后，恶性口腔组织的光谱明显强于正常口腔黏膜组织的光谱。然而，在470 nm发射时，恶性口腔组织的光谱明显弱于正常口腔黏膜组织的光谱。计算并配对了基于每个样品在±5 nm峰面积内330 nm与470 nm发射相对强度比值的诊断算法。比值直方图显示，使用300 nm激发波长可以区分组织学上肿瘤性口腔组织和正常口腔黏膜组织。恶性或癌前口腔样品的平均比值显著高于正常口腔黏膜样品（p < 0.001）。这项离体研究表明，荧光光谱法可能有助于区分恶性或癌前口腔组织与正常口腔黏膜。
