Simple method for simultaneous determination of halothane, enflurane, and isoflurane in Krebs solution using capillary gas chromatography.

A simple gas chromatography (GC) method for the simultaneous determination of halothane, enflurane, and isoflurane in Krebs buffer solution has been developed. The method utilizes methylene chloride as the internal standard and liquid-liquid extraction using chloroform as the solvent. The method demonstrated excellent recovery (100%) of each component and a linear calibration range of 100-700, 100-800, and 300-1,400 micrograms/mL for halothane, isoflurane, and enflurane, respectively. Intra-day accuracy and precision had an error and coefficient of variation of less than 5.1% and 2.7%, respectively.