Yang Q, Atanasov P, Wilkins E
Department of Chemical and Nuclear Engineering, University of New Mexico, Albuquerque 87131-1341, USA.
Biomed Instrum Technol. 1997 Jan-Feb;31(1):54-62.
A new surface-process technology employing electrochemical fixation of a bioactive substance (enzyme and heparin) to a sensor electrode was developed to provide biocompatability and functionality. The fabrication process includes electroentrapment of glucose oxidase and heparin on a platinum electrode by using 1,3-phenylenediamine codeposition. Electrochemically grown 1,3-phenylenediamine was also used as the outer coating of the sensor's enzyme electrode in order to extend the linear range. The sensor shows a sensitivity of 3 nA/mM and a linear range from 40 to 400 mg/dL at 37 degrees C when tested in whole blood. This sensor is characterized by a fast response. The sensor shows a minimum change in its performance when stored inactive in buffer for 12 weeks. When tested at physiologic glucose levels, the sensor demonstrates satisfactory low interference from common interfering substances. This technology seems promising for the preparation of implantable intravascular biosensors.
一种新的表面处理技术被开发出来，该技术通过将生物活性物质（酶和肝素）电化学固定到传感器电极上，以提供生物相容性和功能性。制造过程包括利用1,3 - 苯二胺共沉积将葡萄糖氧化酶和肝素电捕获在铂电极上。电化学生长的1,3 - 苯二胺也被用作传感器酶电极的外涂层，以扩展线性范围。在全血中进行测试时，该传感器在37摄氏度下显示出3 nA/mM的灵敏度和40至400 mg/dL的线性范围。该传感器的特点是响应迅速。当在缓冲液中无活性储存12周时，传感器的性能变化最小。在生理葡萄糖水平下进行测试时，该传感器对常见干扰物质的干扰表现出令人满意的低水平。这项技术对于制备可植入血管内生物传感器似乎很有前景。
