Arce A, Castillón P, Cardinali D P, Esquifino A I
Departamento de Fisiología, Facultad de Medicina, Universidad de Buenos Aires, Argentina.
J Auton Nerv Syst. 1997 Feb 17;62(3):155-62. doi: 10.1016/s0165-1838(96)00120-8.
The local autonomic denervation of rat submaxillary lymph nodes was achieved by a unilateral sympathetic superior cervical ganglionectomy and/or the unilateral section of chorda tympani (that resulted in ipsilateral parasympathetic decentralization of the submandibular territory). This study was performed to determine: (1) whether local sympathetic and/or parasympathetic denervation of rat submaxillary lymph nodes brought about changes in lymph node cellularity, natural killer activity and lipopolysaaccharide (LPS)- and concanavalin A (Con A)-induced cell proliferation in Freund's adjuvant-injected rats; (2) whether the effect of the immunosuppressive drug cyclosporine in rat submaxillary lymph nodes was affected by a single or combined unilateral ganglionectomy plus decentralization. A unilateral ganglionectomy, or the combination of ganglionectomy plus decentralization, performed 7 days earlier, decreased significantly cellularity in ipsilateral submaxillary lymph nodes, while a unilateral decentralization failed to affect it. Natural killer activity increased ipsilaterally after ganglionectomy or decentralization, and decreased after the combined surgical procedure. LPS-induced cell proliferation augmented significantly after ganglionectomy or decentralization, while Con A-induced T lymphocyte proliferation remained unaffected. In the sham-operated side, cyclosporine decreased submaxillary lymph node cell number and natural killer activity, while it increased the proliferative response to LPS. The depressive effect of cyclosporine on lymph node cellularity was no longer observed in ganglionectomized or decentralized lymph nodes, but was found after the combined surgical denervation. Decentralization, or decentralization plus ganglionectomy, blunted the depressive effect of cyclosporine on natural killer activity. The stimulatory effect of cyclosporine on lymphocyte proliferation induced by LPS was reversed both by ganglionectomy or by decentralization and was suppressed by the combined surgical procedure. Neither treatment affected Con A-induced proliferation of T lymphocytes. The results further indicate that an appropriate sympathetic and parasympathetic local environment may be needed for immunomodulation, as well as for cyclosporine activity in lymphoid tissue.
通过单侧交感神经颈上神经节切除术和/或鼓索神经单侧切断术（导致同侧下颌下区域副交感神经去神经支配）实现大鼠下颌下淋巴结的局部自主神经去神经支配。本研究旨在确定：（1）大鼠下颌下淋巴结的局部交感神经和/或副交感神经去神经支配是否会导致弗氏佐剂注射大鼠的淋巴结细胞数量、自然杀伤活性以及脂多糖（LPS）和刀豆蛋白A（Con A）诱导的细胞增殖发生变化；（2）免疫抑制药物环孢素对大鼠下颌下淋巴结的作用是否受到单侧神经节切除术加去神经支配单一或联合操作的影响。提前7天进行的单侧神经节切除术或神经节切除术加去神经支配的联合操作，显著降低了同侧下颌下淋巴结的细胞数量，而单侧去神经支配则未产生影响。神经节切除术后或去神经支配后，同侧自然杀伤活性增加，联合手术操作后则降低。神经节切除术后或去神经支配后，LPS诱导的细胞增殖显著增强，而Con A诱导的T淋巴细胞增殖则不受影响。在假手术侧，环孢素降低了下颌下淋巴结细胞数量和自然杀伤活性，同时增加了对LPS的增殖反应。在神经节切除或去神经支配的淋巴结中，不再观察到环孢素对淋巴结细胞数量的抑制作用，但在联合手术去神经支配后仍可发现。去神经支配或去神经支配加神经节切除术减弱了环孢素对自然杀伤活性的抑制作用。环孢素对LPS诱导的淋巴细胞增殖的刺激作用在神经节切除术后或去神经支配后均被逆转，并被联合手术操作所抑制。两种处理均未影响Con A诱导的T淋巴细胞增殖。结果进一步表明，免疫调节以及环孢素在淋巴组织中的活性可能需要适当的交感神经和副交感神经局部环境。
