Esquifino A I, Rosenstein R E, Stern J, Cardinali D P
Departamento de Bioquímica, Facultad de Medicina, Universidad Complutense, Madrid, Spain.
Eur J Pharmacol. 1991 May 17;197(2-3):161-5. doi: 10.1016/0014-2999(91)90516-s.
In order to examine whether cyclosporine activity in submaxillary lymph nodes is dependent on sympathetic modulation, rats received a unilateral superior cervical ganglionectomy together with a contralateral sham-operation. Two weeks later, cyclosporine (5 or 20 mg/kg per day s.c.) was injected for five days. Freund's complete adjuvant was injected 1 h before the third injection of cyclosporine and the rats were killed 2 h after the last injection of cyclosporine. A significant increase in ornithine decarboxylase activity in submaxillary lymph nodes was observed two weeks after sympathetic denervation. Cyclosporine decreased enzyme activity in submaxillary lymph nodes on the sham-operated side by 67-77% and by 21-41% on the denervated side (P less than 0.01). The incorporation of [35S]methionine into proteins in ipsilateral submaxillary lymph nodes was increased by unilateral superior cervical ganglionectomy and decreased by cyclosporine to a similar extent in denervated and innervated lymph nodes. Superior cervical ganglionectomy decreased by about 93-95% the norepinephrine content of submaxillary lymph nodes regardless of cyclosporine treatment. The results indicate that an appropriate sympathetic neural environment is needed for cyclosporine to have an effect on ornithine decarboxylase activity in lymphoid tissue.
为了研究颌下淋巴结中环孢素的活性是否依赖于交感神经调节，对大鼠进行单侧颈上神经节切除术并对侧假手术。两周后，皮下注射环孢素（每天5或20mg/kg），持续五天。在第三次注射环孢素前1小时注射弗氏完全佐剂，在最后一次注射环孢素后2小时处死大鼠。交感神经去支配两周后，观察到颌下淋巴结中鸟氨酸脱羧酶活性显著增加。环孢素使假手术侧颌下淋巴结中的酶活性降低67 - 77%，使去神经侧降低21 - 41%（P < 0.01）。单侧颈上神经节切除术使同侧颌下淋巴结中[35S]甲硫氨酸掺入蛋白质增加，而环孢素在去神经和有神经支配的淋巴结中均使这种掺入降低到相似程度。无论环孢素治疗如何，颈上神经节切除术使颌下淋巴结中去甲肾上腺素含量降低约93 - 95%。结果表明，环孢素对淋巴组织中鸟氨酸脱羧酶活性产生影响需要合适的交感神经环境。
