Petrova L Ia, Kazanina G A, Selezneva A A
Prikl Biokhim Mikrobiol. 1977 Jul-Aug;13(4):521-6.
By gel chromatography and polyacrylamide gel disc-electrophoresis the component composition of the culture liquid separated from the mycelium was investigated. After elution from Sephadex G-75 column lipase activity was concentrated in one peak. Out of 13 electrophoretic mobile protein components only one split trioleate. The paper describes a method to isolate lipase from the native solution of the produced which involves enzyme precipitation at 40% saturation of the native solution with ammonium sulphate, dialysis of the resultant concentrate and selective sorption of pigment and protein admixtures on the cellulose anion exchanger. The method maker it possible to obtain a highly purified lipolytic enzyme with a specific activity of up to 15 000 lu/mg protein and to provide its 35--40% yield.
通过凝胶色谱法和聚丙烯酰胺凝胶圆盘电泳法，对从菌丝体中分离出的培养液的成分组成进行了研究。从葡聚糖凝胶G - 75柱上洗脱后，脂肪酶活性集中在一个峰中。在13种电泳移动蛋白组分中，只有一种能分解三油酸甘油酯。本文描述了一种从所产生的天然溶液中分离脂肪酶的方法，该方法包括用硫酸铵将天然溶液饱和度调节至40%时进行酶沉淀，对所得浓缩物进行透析，以及在纤维素阴离子交换剂上对色素和蛋白质混合物进行选择性吸附。该方法能够获得比活性高达15000 lu/mg蛋白质的高度纯化的脂肪分解酶，并能提供35 - 40%的产率。
