Ohta Y, Watanabe Y, Tabata T, Oda M, Hayashi Y, Endo Y, Tanaka M, Sasaki T
Department of Surgery I, School of Medicine, Kanazawa University, Japan.
Br J Cancer. 1997;75(4):512-5. doi: 10.1038/bjc.1997.89.
We assessed the inhibitory action of TNP-470 on lymph node metastasis in a metastatic model system using athymic nude mice. Mice were injected subcutaneously with 5 x 10(6) HT-1080 cells in the right groin. TNP-470 (10, 30 and 100 mg kg-1) was injected subcutaneously nine times in total every other day from the 7th day after tumour inoculation. Axillar and inguinal lymph nodes were dissected, and DNA was extracted 5 weeks after tumour inoculation. Specific detection of a human beta-globin-related sequence in metastasized human tumour cells in nude mice was done by the polymerase chain reaction (PCR) technique and analysed by Southern blotting. Anti-tumour effects on primary sites were seen only in the 100 mg kg-1 treatment group. Lymph node metastasis of transplanted HT-1080 cells was seen in all mice of the no treatment group (5/5). On the other hand, incidences of lymph node metastasis in treated mice were 2/4 mice (100 mg kg-1, 2/5 mice (30 mg kg-1) and 4/5 mice (10 mg kg-1). The inhibition ratios of lymph node metastasis were 82.3% at 10 mg kg-1, 97.2% at 30 mg kg-1 and 97.5% at 100 mg kg-1 respectively. This agent may be useful to inhibit lymph node metastasis.
我们在使用无胸腺裸鼠的转移模型系统中评估了TNP - 470对淋巴结转移的抑制作用。将5×10⁶个HT - 1080细胞皮下注射到小鼠右腹股沟。从肿瘤接种后第7天开始，每隔一天皮下注射TNP - 470（10、30和100mg/kg），共注射9次。在肿瘤接种5周后，解剖腋窝和腹股沟淋巴结并提取DNA。通过聚合酶链反应（PCR）技术对裸鼠转移性人肿瘤细胞中的人β - 珠蛋白相关序列进行特异性检测，并通过Southern印迹法进行分析。仅在100mg/kg治疗组中观察到对原发部位的抗肿瘤作用。未治疗组的所有小鼠（5/5）均出现移植的HT - 1080细胞的淋巴结转移。另一方面，治疗组小鼠的淋巴结转移发生率分别为：2/4只小鼠（100mg/kg）、2/5只小鼠（30mg/kg）和4/5只小鼠（10mg/kg）。淋巴结转移的抑制率分别为：10mg/kg时为82.3%，30mg/kg时为97.2%，100mg/kg时为97.5%。该药物可能对抑制淋巴结转移有用。
