Krylov B V, Podzorova S A, Vilin Iu Iu
Fiziol Zh Im I M Sechenova. 1996 Jul;82(7):1-10.
The kinetics of inactivation of TTX-sensitive and TTX-resistant sodium channels in the excitable membrane of rat dorsal root ganglion cells were studied using whole-cell recording technique with the presence of two different pH buffers, Tris and Hepes. It is shown that Tris ions irreversibly interact with inactivation system, accelerating the decaying phase of sodium current. The buffer regulates the gating machinery of two types of the channels. It makes the second order properties more pronounced. On the contrary, the characteristics of inactivation process in Hepes are more close to that we register immediately after the rupture of the cell membrane. We suppose that Hepes buffer is more adequate for investigation of sodium channel inactivation processes.
利用全细胞记录技术，在两种不同的pH缓冲液Tris和Hepes存在的情况下，研究了大鼠背根神经节细胞可兴奋膜中TTX敏感和TTX抗性钠通道的失活动力学。结果表明，Tris离子与失活系统发生不可逆相互作用，加速了钠电流的衰减阶段。该缓冲液调节两种类型通道的门控机制，使二级特性更加明显。相反，Hepes中失活过程的特征更接近于我们在细胞膜破裂后立即记录到的情况。我们认为Hepes缓冲液更适合用于研究钠通道失活过程。
